新疆棉铃虫病原微生物分离及其高效杀虫微生物的筛选  

Isolation of Pathogentic Miroorganism of Helicoverpa armigera and Screening of the High Efficient Insecticidal Microbe

作　　者：史应武 赵思峰 李国英 王钦英 孙庆辉 翁晓梅 

机构地区：[1]新疆兵团绿洲生态实验室,新疆石河子832003 [2]新疆兵团农六师农业技术推广中心,新疆五家渠831300

出　　处：《石河子大学学报（自然科学版）》2003年第2期115-117,共3页Journal of Shihezi University(Natural Science)

摘　　要：从新疆不同生态区自然死亡的980头棉铃虫上分离到360株细菌和12株病毒,经鉴定,其中气杆菌344株,球孢芽孢杆菌8株,粘质赛氏杆菌6株,苏云金芽孢杆菌2株,HaNPV病毒12株,依次占分离物的92.5%、2.2%、1.6%、0.5%和3.2%。从中选出19株细菌、1株HaNPV进行生物测定,结果在供试菌株中以35号和360号菌株及HaNPV毒力最强,其感染棉铃虫后的死亡率分别为80.0%、70.0%、70.0%,对其进行不同浓度的试验,经回归分析,2株苏云金芽孢杆菌35号、360号和1株HaNPV对棉铃虫3龄幼虫的毒力与浓度有高度相关性(r>0.900)。From a total of 980 samples which naturally died larvae of Helicoverpa armigera were collected from several ecological regions of Xinjiang,360 bacteial isolates and 12 virus isolates were isolated.Among them, there were Enterobacter 344,Bacillus sphaericus 8, Serratia marcescens 6, bacillus thuringiensis (Bt) 2 and Helicoverpa armigera nucleoployhedrosis virus (HaNPV) 12 and they were ranged 92.5%, 2.2%, 1.6%, 0.5% and 3.2% of total isolates respectively. Toxicity of typical isolates of 19 bacterial isolates and 1 HaNPV isolates was determined by bioassay. The result shows that 35,360 bacterial isolates and HaNPV have a best toxicity in test isolates and the death rate of infected larvae of H. armigera were 80.0%,70.0% and 70.0% respectively.Toxicity of the different concentration of 35,360 and HaNPV isolates were determined. By regression analysis, toxticy of 2 Bt and HaNPV against 3 instar larvae of H.armigera correlate with the concentration (r>0.900). 

关 键 词：新疆 棉铃虫 病原微生物 分离 高效杀虫微生物 苏云金芽孢杆菌 菌株筛选 

分 类 号：S435.622.3[农业科学—农业昆虫与害虫防治] S482.39[农业科学—植物保护]