环胞霉素A抑制神经肽Y诱导大鼠心肌细胞肥大效应  

Cyclosporine A suppresses the hypertrophic effect of neuropeptide Y in rat cardiomyocytes

作　　者：黄少华[1] 陈敏生[1] 李晓云[2] 李荧辉[3] 董颀[3] 

机构地区：[1]广州医学院第一附属医院心脏病研究室,广东广州510182 [2]广州医学院第二附属医院心内科,广东广州510260 [3]航天医学工程研究所,北京100000

出　　处：《中国病理生理杂志》2003年第7期935-938,共4页Chinese Journal of Pathophysiology

基　　金：广东省自然科学基金项目资助 (No .980 4 6 6 )

摘　　要：目的 :观察Ca2 + /CaM依赖的钙调神经磷酸酶 (CaN)抑制剂环胞素A(CsA)对神经肽Y诱导心肌细胞肥大效应的影响。方法 :用神经肽Y(NPY)刺激Wistar乳鼠心肌细胞 ,并用环胞素A加以干预。应用氚 -亮氨酸([3 H]-Leu)掺入法测定心肌细胞蛋白质合成速率、RT -PCR法测心肌细胞c -junmRNA表达。结果 :(1)心肌细胞氚 -亮氨酸 ([3 H]-Leu)掺入量测定 :与对照组相比 ,NPY 10nmol/L组氚 -亮氨酸 ([3 H]-Leu)掺入量有所增高 ,但与对照组比无显著差别 ,而NPY 10 0nmol/L组心肌细胞氚 [3 H]-Leu掺入量较对照组明显增高 (P <0 0 5 )。CsA组和对照组相比无显著差别。 (2 )心肌细胞内c -junmRNA表达 :NPY组心肌细胞c -junmRNA的RT -PCR产物量明显高于对照组和CsA组 (P <0 0 1) ,对照组和CsA组间无显著差别。结论 :NPY刺激心肌细胞蛋白质合成增加、心肌细胞原癌基因 (肥大早期反应基因 )c -junmRNA表达 ,提示NPY可诱导心肌细胞肥大 ;CaN抑制剂CsA可阻断NPY上述效应 ,说明Ca2 +AIM: To investigate the effect of cyclosporine A on rat cardiomyocyte hypertrophy caused by neuropeptide Y (NPY). METHODS: Cardiomyocytes of neonatal Wistar rats were cultured with NPY or NPY together with CsA. For assessing protein synthesis rate and c-jun mRNA expression in cardiomyocytes, the methods of [ 3H]-Leu incorporation and RT-PCR were used. RESULTS: (1) [ 3H]-Leu incorporation in cardiomyocytes: [ 3H]-Leu incorporation in NPY (10 nmol/L) group was higher than that in control group, but there were no distinct changes between two groups. To compare with control group, [ 3H]-Leu incorporation in NPY (100 nmol/L) group were increased significantly ( P<0.05 ). There was no significant change between control group and CsA group; (2) c-jun mRNA expression in cardiomyocytes: RT-PCR production of c-jun mRNA in NPY group was enhanced considerably compared with CsA group and control group ( P<0.01 ). There was no significant change between CsA group and control group. CONCLUSIONS: NPY can induce cardiomyocyte hypertrophy. Cyclosporine A (inhibitor of CaN) can blunt the effect of NPY, suggesting that the Ca 2+ /CaM-dependent calcineurin (CaN) signaling pathway plays an important role in it.

关 键 词：神经肽Y 环胞菌素 心肌 肥大 

分 类 号：R363[医药卫生—病理学]