糖尿病早期角膜组织的超微变化  

Changes of corneal ultrastructure in early diabetic rats

作　　者：陈剑[1] 黄建艳[1] 唐福星[2] 郭祖文[2] 赵松滨[1] 王彦平[3] 吴春云[4] 

机构地区：[1]暨南大学第一附属医院眼科,广东广州510630 [2]暨南大学电镜室,广东广州510630 [3]暨南大学医学院病理生理教研室,广东广州510630 [4]暨南大学人体寄生虫学教研室,广东广州510630

出　　处：《中国病理生理杂志》2003年第7期942-945,共4页Chinese Journal of Pathophysiology

摘　　要：目的 :了解早期糖尿病角膜组织的超微变化 ,探讨糖尿病角膜病变的发病机制。方法 :选取SD雄性大鼠 4 0只 ,随机分为糖尿病组和正常对照组 ,前者以链脲佐菌素诱导成糖尿病模型。分别于 6、8、10、12周取角膜 ,扫描电镜及透射电镜下观察其超微改变。结果 :各个观察时点糖尿病大鼠角膜上皮和内皮细胞水肿 ,胞浆内线粒体增多和肿胀 ,随着病程进度而明显 ;角膜内皮的破坏从周边开始 ,逐渐向中央发展 ;成模后第 10周开始出现基质层胶原纤维排列紊乱 ,部分呈格子状排列 ,有断裂现象。结论 :糖尿病性角膜病变超微结构的改变导致了角膜功能异常 ,这可能与高血糖时物质代谢异常有关。AIM: To investigate the changes of ultrastructure in early diabetic rat cornea and the pathogenesis of diabetic keratopathy. METHODS: 20 Sprague-Daxley rats were sacrificed 6, 8, 10 and 12 weeks after induction of diabetes mellitus by streptoxotocin. 20 untreated rats at the same age were used as normal controls and were sacrificed at the same intervals. The ultrastructures of cornea were observed with transmission electronic microscopy and scanning electron microscopy. RESULTS: During the experimental period, the corneal ultrustructure of diabetic rats showed that epithelial and endothelial cells were swollen, the mitochondrions in the cytoplasm were swollen and increased. The collagen fibers appeared in disarrangement 10 weeks after streptoxotocin treatment. The endothelial of cornea was damaged from the periphery to the center gradually. CONCLUSION: The ultrastructural changes of cornea leads to dysfunction in streptoxotocin-induced diabetic rats, which may be related to the abnormal metabolism in hyperglycemia condition.

关 键 词：糖尿病 大鼠 角膜 超微结构 

分 类 号：R363[医药卫生—病理学]