硫酸肝素对人肝癌HepG2细胞系增殖和凋亡的影响  

Effects of sulphated heparin on proliferation and apoptosis of human hepatocellular carcinoma cell line (HepG2)

作　　者：夏克勤[1] 张海伟[2] 郑佩娥[2] 

机构地区：[1]暨南大学医学院医学院院部 [2]暨南大学医学院病理教研室

出　　处：《中国病理生理杂志》2003年第7期961-963,T005,共4页Chinese Journal of Pathophysiology

基　　金：广东省卫生厅资助;"五个一"兴教工程基金资助

摘　　要：目的 :探讨硫酸肝素对人肝癌HepG2细胞系增殖和凋亡的影响。方法 :体外观察硫酸肝素作用于人肝癌细胞系 (HepG2 )的细胞增殖、凋亡效应和细胞ras基因表达的变化关系。结果 :硫酸肝素能下调细胞HepG2的增殖能力及其细胞ras基因蛋白的表达并上调其细胞的凋亡率。结论 :硫酸肝素对人肝癌细胞增殖、凋亡的影响与ras基因蛋白介导的信号转导有关。AIM: To explore the effect and the mechanism of sulphated heparin on the proliferation and the apoptosis of human hepatocellular carcinoma cells. METHODS: The human hepatocellular carcinoma cell line (HepG-2) was used to identify the expression of ras gene protein and to study the effect of sulphated heparin on proliferation and the apoptosis in vitro . RESULTS: The sulphated heparin downregulated the ras protein expression and inhibited the cell growth in HepG2 cells. In the presence of sulphated heparin, the apoptosis rate of HepG2 increased. CONCLUSION:The data suggest that the effects of sulphated heparin on the proliferation and the apoptosis of the human hepatocellular carcinoma cell are correlated with the signaling transduction mediated by ras gene protein.

关 键 词：肝肿瘤 增殖 ras蛋白质类 肝素 信号转导 细胞凋亡 

分 类 号：R735.7[医药卫生—肿瘤]