转铁蛋白结合镱跨膜转运及镱对U-87 MG细胞增殖的影响  

Transferrin-bound Yb_2 uptake by U-87 MG cells and effect of Yb on proliferation of the cells

作　　者：王夔[1] 杜秀莲[1] 常彦忠[2] 李荣昌[1] 司徒加怡[3] 孙红哲[3] 钱忠明[2] 

机构地区：[1]北京大学药学院化学生物系,北京100083 [2]香港理工大学应用生物与化学科技学系铁代谢实验室 [3]香港大学化学系

出　　处：《中国病理生理杂志》2003年第7期902-904,共3页Chinese Journal of Pathophysiology

基　　金：香港理工大学研究基金 (A -PC2 3;A -PC98);香港理工大学与北京大学联合培养博士生基金资助项目 ( 2 0 0 0 - 1)

摘　　要：目的 :研究转铁蛋白 /转铁蛋白受体转运系统对转铁蛋白结合镱 (Yb2 Tf)跨膜转运进入神经胶质瘤U- 87MG细胞 ,以及转铁蛋白结合镱和非转铁蛋白结合镱对U - 87MG细胞增殖的影响。方法 :细胞培养及ICP -MS镱测定法。结果 :随Yb2 Tf浓度增加 ,细胞镱摄入量增加。当浓度达 2 μmol/L时 ,细胞摄取镱基本达到饱和状态。细胞摄入镱量也随镱 :apoTf摩尔比增大而增加 ,当摩尔比达到 1.5时 ,摄入量达到最高水平。 0 .4 μmol/LYb2 Tf可显著抑制U - 87MG细胞增殖 ,而Yb3 +浓度高达 10 μmol对细胞增殖仅有轻微影响。 结论 :转铁蛋白 /转铁蛋白受体介导的膜转运可能是镱跨越U - 87MG细胞的机制之一。转铁蛋白结合Yb3 +可以有效地抑制U - 87MG细胞的增殖。AIM: To investigate the role of transferrin/transferrin receptor system in transferrin-bound Yb 2 (Yb 2Tf) uptake by U-87 MG cells and the effect of transferrin-bound and -free Yb 2 on proliferation of U-87 MG cells. METHODS: Cell culture and ICP-MS measurement of Yb 2. RESULTS: Yb 2Tf uptake by U-87 MG cells increased with the concentrations of Yb 2Tf, and reached saturation as the concentration in the incubation medium was raised to about 2 μmol/L. Also, Yb 2 uptake by the cells increased with increase of the mole ratio (Yb 2: apoTf), reaching a maximum at 1.5 mole ratio. Yb 2Tf in 0.4 μmol/L significantly inhibited proliferation of U-87 MG cells, however, 10 μmol/L Yb 3+ had no significant effect on proliferation of the cells. CONCLUSION: The uptake of Yb 2 by U-87 MG cells might be mediated by transferrin/transferrin receptor system. Transferrin-bound but not transferrin-free Yb 2 could significantly inhibit proliferation of U-87 MG cells.

关 键 词：Yb^3+/Yb2Tf 转铁蛋白/转铁蛋白受体 神经胶质瘤 U-87 MG细胞 

分 类 号：R363[医药卫生—病理学]