虾仁中氯霉素残留量的气相色谱测定  

Determination of residual CAP in shrimps by GC-ECD

作　　者：程晶波[1] 施国华[1] 

机构地区：[1]浙江工业大学职业技术学院环境工程系,浙江杭州310014

出　　处：《福建分析测试》2003年第1期1700-1703,共4页Fujian Analysis & Testing

摘　　要：建立了用配有电子俘获检测器(ECD)的气相色谱仪来测定虾仁中的氯霉素含量的方法。试样用乙酸乙酯提取,正己烷去杂,硅胶柱纯化.BSTFA+TMCS(99:1)试剂硅烷化。以GC-ECD方式测定,外标法定量。当添加水平为0.2×10~9～1.0×10^(-9)μg/kg时,回收率为70%～110%;相对标准偏差为1.0%～5.0%;线性相关系数,r>0.998。A gas chromatography with electronic capture detection (ECD) for determination of chloramphenicol (CAP) residues in shrimps samples has been validated. The samples were extracted with ethyl asctate, defatted with hexane and passed through silica SPE column. The eluted solutions were derivatized with BSTFA + TMCS (99 : 1). The solution was determined with DC-ECD. using external standard calibration. When the added level of the standard were between 0. 2×10-9-1.0× 10-3μg/kg, the recoveries were 50% - 110%, the RSD were 0. 9% -5. 0% , the correlation coefficient r>0. 998.

关 键 词：虾仁 气相色谱法 氯霉素 外标法 最小检出量 回收率 检出限 

分 类 号：TS254.7[轻工技术与工程—水产品加工及贮藏工程] O657.71[轻工技术与工程—食品科学与工程]