荧光定量逆转录聚合酶链反应检测高苯丙氨酸对2种神经元发育相关基因表达的影响  

Expression of two neuron developmental associated genes induced by hyperphenylalanine with real time quantitative RT-PCR

作　　者：张惠文[1] 顾学范[1] 

机构地区：[1]上海第二医科大学附属新华医院上海市儿科医学研究所内分泌遗传代谢病研究室,200092

出　　处：《中华检验医学杂志》2003年第5期273-276,T001,共5页Chinese Journal of Laboratory Medicine

基　　金：国家自然科学基金资助项目 ( 3 0 0 70 2 41)

摘　　要：目的　应用实时荧光定量逆转录聚合酶链反应 (RT PCR) ,研究高苯丙氨酸对神经元发育相关基因表达的影响。方法　在体外培养 3d的原代胚鼠大脑皮层神经元中 ,加入高苯丙氨酸诱导 12h ,用实时荧光定量RT PCR方法 ,检测高苯丙氨酸对胚鼠大脑皮层神经元生长相关蛋白 4 3(GAP 4 3)和鸟苷酸结合蛋白α1(Goα1)基因的影响。结果　胚鼠大脑皮层神经元经高苯丙氨酸诱导 ,其GAP 4 3基因表达较正常对照增高 2 2 5倍 ,Goα1较正常对照降低 3 31倍。结论　高苯丙氨酸可能通过影响GAP 4 3和Goα1基因表达 ,干扰大脑皮层神经元正常生长发育。Objective To determine the expression of two neuron developmental associated genes induced by hyperphenylalanine.Methods Primary embryonal rat cerebral cortical neurons were cultured for three day and cells induced by hyperphenylalanine for 12 hours.Real time quantitative RT-PCR was used to determine the influence of hyperphenylalanine on the expression of GAP-43 and Goα1 genes.Results GAP-43 mRNA was upregulated to 2.25 times and Goα1 was downregulated to 3.31 times by hyperphenylalanine.Conlusion Hyperphenylalanine may interfere the normal development of cerebral cortical neurons through influencing the expression of GAP-43 and Goα1 genes.

关 键 词：高苯丙氨酸血症 荧光定量 逆转录聚合酶链反应 检测 高苯丙氨酸 神经元发育 基因表达 

分 类 号：R446.9[医药卫生—诊断学]