奥沙利铂对A549肺腺癌细胞系生长抑制作用和细胞凋亡的实验研究  

Study of Cell Growth Inhibition and Apoptosis Induced by Oxaliplatin in Human Lung Adenocarcinoma Cell Line-A549

作　　者：郭正丽[1] 吴洁[1] 杨吉成[2] 盛伟华[2] 谢宇峰[2] 李丽娥[2] 

机构地区：[1]苏州大学附属第一医院呼吸内科,苏州215006 [2]苏州大学医学院细胞和分子生物学教研室,苏州215007

出　　处：《苏州大学学报（医学版）》2003年第3期286-288,共3页Suzhou University Journal of Medical Science

摘　　要：目的　观察奥沙利铂对肺腺癌细胞系A5 4 9的药物敏感性和诱导细胞凋亡作用。方法　将不同浓度的奥沙利铂与A5 4 9作用 2 4、36、4 8h ,用MTT法计算细胞生长抑制率 ,流式细胞仪检测细胞凋亡率。结果　奥沙利铂对A5 4 9的细胞生长抑制率随作用浓度和作用时间的增加而增大。 12 .5、2 5、5 0、10 0 μg/ml奥沙利铂对A5 4 9作用 2 4h ,细胞凋亡率分别为 35 %、13.6 %、2 .6 %、1.4 %。结论　奥沙利铂对A5 4 9细胞有生长抑制作用 ,呈剂量依赖性和时间依赖性 ;能引起细胞凋亡 ,细胞凋亡率呈剂量依赖性 ,与细胞生长抑制率呈正相关关系 (P <0 .0 1)。Objective To research in vitro chemosensitivity and apoptosis induced by oxaliplatin in human lung adenocarcinoma cell line-A549. Methods A549 was incubated with oxaliplatin of virous concentration , cell growth inhibition was assesed by MTT colorimetric assay and apoptotic rate was detected by FCM. Results At dose of 100,50,25,12.5 μg/ml, oxaliplation apparently inhibited growth of A549 cell, FACS can showed apoptotic rate was 35%,13.6%,2.6%,1.4% for 24 h treatment at dose of 100,50,25,12.5 μg/ml respectively. Conclusion The cell growth inhibition of oxaliplatin on A549 is dose-dependent and time-dependent, oxaliplatin can induce apoptosis in A549 cell which is dose-dependent. There is a positive linear correlation between apoptosis assessed by FACS can and growth inhibition rates by MTT( P < 0.01).

关 键 词：肺腺癌 A549 奥沙利铂 细胞凋亡 药物敏感性 治疗 流式细胞仪 实验研究 

分 类 号：R734.2[医药卫生—肿瘤] R73－361[医药卫生—临床医学]