9-顺-维甲酸对非小细胞肺癌CyclinD1、Cdk4表达的影响  

Effects of9-cis-retinoic acid on expression of CyclinD1and Cdk4in non-small cell lung cancer

作　　者：任桂杰[1] 刘志方[1] 丁磊[1] 胡国强[2] 胡晓燕[1] 于雪艳[1] 

机构地区：[1]山东大学医学院生化与分子生物学研究所 [2]山东大学齐鲁医院胸外科

出　　处：《山东大学学报（医学版）》2003年第3期235-237,共3页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金资助项目(39770730)

摘　　要：目的:研究9-顺-维甲酸对非小细胞肺癌A549、PG、SPC-A1的细胞周期蛋白D1(cyclinD1)、细胞周期蛋白依赖性蛋白激酶4(Cdk4)表达的影响,探讨其抑制非小细胞肺癌生长与影响CyclinD1、Cdk4表达的相关性。方法:采用半定量逆转录PCR方法,分析细胞周期因子CyclinD1、Cdk4表达的变化。结果:9-顺-维甲酸既可以降低PG、SPC-A1的CyclinD1表达(P<0.01),又可以降低PG、A549的Cdk4表达(P<0.01)。结论:9-顺-维甲酸对非小细胞肺癌生长的抑制作用,与其调控细胞周期因子CyclinD1、Cdk4的表达密切相关。Objective:To study the effects of9-cis-retinoic acid(9-cis-RA)on the expression of CyclinD1and Cdk4in non-small-cell lung cancer PG,A 549 and SPC-A1,and to investigate the correla-tion between inhibition of cell growth by9-cis-RA and interference with the expression of CyclinD1and Cdk4in non-small-cell lung cancers.Method s:RT-PCR was used to analyze the changes in the expression of CyclinD1and Cdk4of PG,A 549 and SPC-A1.Results:9-cis-RA can not only decrease the transcription of CyclinD1of PG and SPC-A1(P<0.01),but also decrease the transcription of Cdk4of PG and A 549 (P<0.01).Conclu sion:The growth-inhibitory effect of9-cis-RA on non-small-cell lung cancers was tightly correlated with its mediation of the transcription of CyclinD1and Cdk4.

关 键 词：维甲酸 癌 非小细胞肺 细胞周期蛋白D1 细胞周期蛋白依赖性蛋白激酶4 

分 类 号：Q562[生物学—生物化学] R730.1[医药卫生—肿瘤]