肝性脑病大鼠肝和脑组织一氧化氮及一氧化氮合酶的研究  

Study on nitric oxide and nitric oxide synthase in liver and brain tissues of experimental hepatic encephalopathy rats

作　　者：邵丽华[1] 汪心婷[1] 马立宪[2] 孙淑爱[1] 王淑娥[1] 潘玉珍[1] 

机构地区：[1]山东大学公共卫生学院卫生检验研究所 [2]山东大学医学院传染病学教研室

出　　处：《山东大学学报（医学版）》2003年第3期257-259,共3页Journal of Shandong University:Health Sciences

基　　金：山东省卫生厅科研基金资助课题(1999CA1CAA6)

摘　　要：目的:探讨一氧化氮(NO)与肝性脑病(HE)的关系。方法:用硫代乙酰胺(TAA)制造大鼠HE模型,35只大鼠随机分为2组,HE模型组25只,正常对照组10只。HE模型组第1天均腹腔注射TAA300mg·kg-1,第2～4天,每日分别注射TAA150mg·kg-1,直至出现HE症状。正常组每日注射等量生理盐水。采用分光光度法测定两组大脑、小脑、肝组织中NO含量和一氧化氮合酶(NOS)活性。结果:HE模型组大脑、小脑、肝组织中NO含量与正常组比较明显升高,差异均有统计学意义(P<0.05,P<0.05,P<0.01);NOS活性与正常组比较明显升高,差异均有统计学意义(P<0.05,P<0.05,P<0.01);HE模型组大脑、小脑、肝组织中NO含量与NOS活性呈正相关(r=0.7284,r=0.8220,r=0.7010;P<0.001)。结论:NO可能是肝性脑病的重要影响因素之一。Objective:To research the relationship of nitric oxide(NO)and hepatic encephalopa-thy(HE).Methods:Experimental HE model was induced in rats with thioacetamide(TAA).35rats were randomly assigned to two groups:25rats in HE group and10rats in control group.By ab-dominocentesis,TAA300mg ·kg -1 were given to the rats of HE group at1st day,and from2nd to4th day,the dosage of TAA was150mg ·kg -1 per day until the symptoms of HE were appeared.Normal saline was given to the rats of control group.NO levels and nitric oxide synthase(NOS)activity in cerebrum,cerebellum and liver were measured by spectrophotometry in each group of above.Results:Compared to control group,NO level in cerebrum,cerebellum and liver evidently increased in HE rats(P<0.05,P<0.05,P<0.01).NOS activity in these tissues of HE rats increased too(P<0.05,P<0.05,P<0.01).Relative analysis demonstrated NO level had significant correlation with NOS activity in cere-brum,cerebellum and liver of HE rats(r=0.7284,r=0.8220,r=0.7010;P<0.001).Conclusion:It is sug-gested that NO be one of important causes of HE.

关 键 词：肝性脑病 一氧化氮 一氧化氮合酶 大鼠 近交系 

分 类 号：R575.3[医药卫生—消化系统]