肝癌患者外周血中白蛋白mRNA检测的意义  

Detection of albumin messenger RNA in peripheral blood of patients with hepatocellular carcinoma

作　　者：孙国瑞[1] 王占民[1] 吴小鹏[1] 董孝媛[2] 

机构地区：[1]山东大学齐鲁医院普外科 [2]山东大学齐鲁医院血液科

出　　处：《山东大学学报（医学版）》2003年第3期299-301,307,共4页Journal of Shandong University:Health Sciences

基　　金：山东省科技厅资助课题(963000053)

摘　　要：目的:探讨肝癌患者外周血中白蛋白mRNA在血行播散中的意义。方法:抽取30例肝癌患者外周静脉血5ml,应用RT-PCR技术对血中白蛋白mRNA进行扩增检测。结果:敏感性实验中,健康人血中存在102以上个肝癌细胞时可测出。30例HCC患者中,白蛋白mRNA的阳性率为60%(18/30);白蛋白mRNA的检出率与肝癌的TNM分期、肿瘤大小、有无门脉癌栓及有无肝外转移有显著的相关性(P<0.05),而与血清AFP值无关(P>0.75)。结论:白蛋白mRNA是反映原发性肝癌患者血中是否存在肝癌细胞的灵敏指标。Objective:To determine whether albumin messenger RNA(mRNA),a specifically hepatocyte-expressed gene,could be a marker of metastasis in hepatocellular carcinoma.Methods:Pe-ripheral blood samples were obtained from30patients with hepatocellular carcinoma (HCC)and RT-PCR technique was used to detect the human albumin mRNA in the blood.Results:In the sensitivity test,10 2 hepatocytes mixed into health controls' blood can be detected.Of the30HCC patients,18(60%)patients had positive albumin mRNA.The presence of albumin mRNA correlated with the TNM clinical stages,the size of tumor,extrahepatic metastasis or with portal vein carcinothrombosis(P<0.05).There was no significant correlation between the positive rate of albumin mRNA and the serumα-feto-protein(AFP)(P>0.05).Conclu sion:The positive detection of albumin mRNA in the peripheral blood can indicate the presence of hematogenous spreading metastasis in patients with HCC.

关 键 词：肝肿瘤 白蛋白MRNA 聚合酶链反应 

分 类 号：R735.7[医药卫生—肿瘤] R73－37[医药卫生—临床医学]