KGF基因在COS7细胞中的表达及其活性检测  

KGF gene expression in COS7 cell and KGF bioactivity assay

作　　者：傅成波[1] 赵蓓蕾[1] 王宝勤[1] 苑晓玲[1] 罗庆良[1] 熊国林[1] 

机构地区：[1]军事医学科学院放射医学研究所,北京100850

出　　处：《军事医学科学院院刊》2003年第3期172-173,192,共3页Bulletin of the Academy of Military Medical Sciences

摘　　要：目的 :利用COS7细胞表达角质细胞生长因子 (KGF)并检测其活性。方法 :利用脂质体把KGF基因转入COS7细胞中 ,再用Western印迹和ELISA方法检测KGF蛋白的表达 ,然后用MTT法检测其活性。结果 :KGF基因在COS7中得到了表达 ,并对小鼠肠上皮细胞 (IEC_6 )具有刺激增殖作用。结论 :KGF有望成为肠型放射病治疗的新型药物。Objective:To express KGF in COS7 cells and assay its biological activity.Methods:KGF gene was transferred to COS7 by LipofectAMINE and its expression was assayed by Western_blot and ELISA method and its bioactivity was assayed by MTT. Results:KGF gene was expressed in COS7 and KGF was able to stimulate IEC_6 cells.Conclusion:KGF is hopeful to be a new therapeutic drug for intestinal type of radiation damage.

关 键 词：角质细胞生长因子 COS7细胞 基因表达 脂质体 印迹法 蛋白质 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]