SARS冠状病毒及相关病毒的分子系统学分析  

MOLECULAR PHYLOGENETIC ANALYSIS OF SARS CORONAVIRUS AND RELATED VIRUSES

作　　者：张原[1] 齐一琳[1] 洪泂[1] 郑光宇[1] 阮力[2] 郑楠[1] 

机构地区：[1]北京师范大学生命科学学院 [2]中国疾病预防控制中心病毒病预防控制所,北京100052

出　　处：《北京师范大学学报（自然科学版）》2003年第3期402-406,共5页Journal of Beijing Normal University(Natural Science)

摘　　要：根据SARS冠状病毒及其相关病毒的基因组核酸序列和 3种不同蛋白质序列 ,应用最大简约法和最小进化法重建系统发育树 ;并对SARS冠状病毒的 11个推测蛋白质 (ORF)做BLAST分析。结果表明 ,SARS冠状病毒和鼠肝炎病毒———牛冠状病毒分支构成姊妹群 .其单系群性质得到强有力的统计学支持 .这暗示了SARS的爆发可能源自种间屏障的突破事件 ,该病毒天然宿主可能为猪、牛或鼠 .SARS冠状病毒与已知的人冠状病毒分属冠状病毒科的不同分支 ,因此致病机制可能有很大不同 .3个基因的系统树分支格局的一致性表明 :SARS冠状病毒这 3个主要基因与其他冠状病毒间不存在重组 ,但全部Based on three different protein sequences and complete genome sequences, phylogenetic trees are reconstructed using maximum parsimony and minimum evolution. BLAST is used for further analysis of the genome of SARS coronavirus. The results show that SARS coronavirus is the sister group of Murine hepatitis virus Bovine coronavirus clade (M B clade). The monophyly of SARS and M B received strong statistical support. This indicates that the outbreak of SARS is due to a event of breaking species barrier. The natural hosts of SARS may be rats, cattle or pigs. SARS coronavirus and Human coronavirus belong to different monoclade of coronaviruses. This suggestes that the two viruses have different pathogenic mechanism. The same branching pattern obtained from different proteins shows that there is no recombination among SARS coronavirus and other known coronaviruses of these 3 major genes. However, the result of BLAST analysis shows that there may be recombinations among SARS coronavirus and other viruses of some small fragments of the genome.

关 键 词：SARS冠状病毒 相关病毒 分子系统学 基因组核酸序列 蛋白质序列 致病机制 严重急性呼吸道综合症 非典型肺炎 

分 类 号：R373.1[医药卫生—病原生物学] R394[医药卫生—基础医学]