甜菜多粘菌拮抗放线菌的筛选及其防治丛根病效果的检测  

Screening of actinomyces against Polymyxa betae and detection of effectiveness on rhizomania disease

作　　者：王琦[1] 王慧敏[1] 于嘉林[2] 刘仪[2] 

机构地区：[1]中国农业大学农学与生物技术学院 [2]中国农业大学农业生物技术国家重点实验室,北京100094

出　　处：《中国农业大学学报》2003年第3期56-60,共5页Journal of China Agricultural University

摘　　要：在甜菜丛根病重病田选取轻病株 ,分离其根面和根内放线菌 ,2 6个分离物有 3株可以拮抗甜菜多糖菌Polymyxabetae。该 3株放线菌经鉴定是链霉菌属 (Streptomyces)的 3个种。拮抗菌的代谢液能够抑制P .betae休眠孢子萌发 ,使游动孢子泳动减缓。甜菜坏死黄脉病毒 (BNYVV)酶联免疫检测 (ELISA)表明 ,拮抗菌处理的根系内BNYVV数量低于对照 ;PCR检测表明 ,对照可扩增出P .betae特异性片段 ,而拮抗菌处理未能扩增出相应片段。本研究表明 3株拮抗菌对P .betae有明显的抑制作用 。actinomyces strains were isolated from the surface and interior parts of lightly infected sugar beet roots, which were samples from heavily infected field. Three isolates were found to be effective on Polymyxa betae and identified as Streptomyces spp. Metabolic solution of the three strains could inhibit sprouting of resting spores and moving of zoospores of P. betae. ELISA of Beet Necrotic Yellow Vein Virus(BNYVV) showed that the number of BNYVV in sugar beet roots inoculated with Streptomyces spp. was less than that of the control. PCR showed that no specificity fragment of P.betae was amplified in sugar beet roots treated with Streptomyces spp., but there were the fragments amplified in that of the control. This study showed that the 3 Streptomyces spp.could inhibit Polymyxa betae, and the number of BNYVV decreased after treated.

关 键 词：甜菜 多粘菌 放线菌 菌种筛选 防治效果 丛根病 检测技术 

分 类 号：S435.663[农业科学—农业昆虫与害虫防治] S432.41[农业科学—植物保护]