急性白血病患儿hTERT基因的表达  

Studies of hTERT Gene Expression in Acute Leukemia Children

作　　者：李熙鸿[1] 王晓阳[1] 李馨筱[1] 刘柏林[2] 张燕[2] 廖清奎[1] 

机构地区：[1]四川大学华西第二医院儿科,成都610041 [2]四川大学华西基础医学与法医学院分子生物学研究室

出　　处：《四川大学学报（医学版）》2003年第3期486-488,共3页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金 (批准号 39970 836)资助

摘　　要：目的　探讨人类端粒酶逆转录酶 ( h TERT)基因表达与儿童急性白血病 ( AL)的关系和临床意义。方法　采用逆转录 -聚合酶链反应 ( RT- PCR)方法检测 K5 62白血病细胞株、40例急性白血病患儿和 10例正常对照的 h TERT基因表达。结果　 K5 62白血病细胞株、3 0例急性淋巴细胞白血病 ( AL L)患者中 2 3例、10例急性髓系细胞白血病 ( AML)患者中 7例有 h TERT基因表达。h TERT基因表达见于 AL 所有亚型 ,其相对表达水平与 AL 的完全缓解 ( CR)率有相关性 ,相对表达水平≥ 1.0的患儿 CR率低及生存期短。结论　 h TERT基因表达与儿童急性白血病发生有关 ,h TERT基因高表达的患者 CR率低、无病生存期短 ,hObjective To explore the gene expression of human telomerase reverse transcriptase (hTERT) in acute leukemia (AL)children and its clinical implication. Methods Expression of hTERT mRNA was detected in K562 leukemia cell line, 40 acute leukemia children (AL) and 10 healthy subjects by reverse transcriptase-polymerase chain reaction (RT-PCR). Results hTERT gene was expressed in K562 cell line, acute lymphoblastic leukemia children (23/30), acute myeloid leukemia children (7/10), and healthy subject (1/10). The expression level of hTERT gene was observed in all subtypes of AL and was related to the prognosis of AL, and the patients with relative level ≥ 1.0 were found to have lower complete remission (CR) rates and disease-free survival. Conclusion hTERT gene expression is associated with the pathogenesis of childhood AL. It can serve as a prognostic indicator in childhood AL.

关 键 词：急性白血病 患儿 HTERT基因 表达 RT-PCR 

分 类 号：R733.71[医药卫生—肿瘤]