婴儿双歧杆菌对小鼠黑色素瘤模型肿瘤组织的靶向性  

Investigation on Tumor-targeting Characteristics of Bifidobacterium Infantis to Melanoma in Mice

作　　者：吴瑜[1] 易成[2] 王树人[1] 张敏 张静 

机构地区：[1]四川大学华西基础医学与法医学院病理生理学教研室,成都610041 [2]四川大学华西医院肿瘤中心 [3]四川大学华西基础医学与法医学院同位素室

出　　处：《四川大学学报（医学版）》2003年第3期435-438,共4页Journal of Sichuan University(Medical Sciences)

基　　金：美国纽约中华医学基金资助

摘　　要：目的　探讨婴儿双歧杆菌对黑色素瘤组织是否具有靶向特性。方法　采用荷瘤鼠尾静脉推注 3H-Td R标记婴儿双歧杆菌示踪分析、组织培养和组织切片革兰氏染色观察婴儿双歧杆菌的瘤组织靶向性。结果　瘤组织内放射性强度持续增强 ,正常组织放射性强度则持续减弱。组织培养显示婴儿双歧杆菌在瘤组织内富集增殖。组织切片显示瘤组织内大量革兰氏染色阳性条杆状紫蓝色深染区 ,而正常组织中却无。Objective To investigate the tumor-targeting of bifidobacterium infantis to melanoma. Methods After bolus administration of bifidobacterium infantis with 3H-TdR tagged, the values of radioactivity in tumor and organs were examined at days 1, 2, 3, 4 and 7. Anaerobic culture and histoglogical observation of tumor and normal organs were taken for the examination of tumor-targeting characteristics of bifidobacterium infantis. Results The radioactivity in melanoma tissue increased progressively,while the radioactivity in normal organs became attenuated with time. The anaerobic culture showed an obvious proliferation of bifidobacterium infantis in tumor tissue. A large part of area was Gram positive in the tumor tissue section, whereas the normal tissue was Gram negative. Conclusion Bifidobacterium infantis has good tumor-targeting characteristics in mice melanoma.

关 键 词：婴儿双歧杆菌 小鼠黑色素瘤模型 肿瘤组织 靶向性 

分 类 号：R73-36[医药卫生—肿瘤]