人参皂甙Rg3对乳腺浸润性导管癌的抗癌作用及其机制  

Effect of Ginsenoside Rg3 on the Progression of Orthotopically Xenotransplanted Human Breast Cancer in Nude Mice and Its Mechanism

作　　者：陈大富 赵扬冰[2] 白绍槐[3] 史宗道[4] 张杰[5] 

机构地区：[1]广州市海军医院普外科,广州510318 [2]四川大学华西医院普外科 [3]四川大学实验动物中心 [4]四川大学华西临床医学院临床流行病学教研室 [5]四川大学华西医院病理科

出　　处：《四川大学学报（医学版）》2003年第3期546-548,共3页Journal of Sichuan University(Medical Sciences)

摘　　要：目的　探讨人参皂甙 Rg3 ( Rg3 )抑制裸鼠原位种植人乳腺浸润性导管癌的作用及其机制。方法　采用移植人乳腺浸润性导管癌的雌性裸鼠 15只 ,随机分为 Rg3组、环磷酰胺 ( CTX)组和对照组 ,分别用 Rg3、CTX及对照剂连续灌胃 5 6d,处死后病理标本用光镜及免疫组化检查。结果　 Rg3组瘤重 ( 0 .84± 0 .2 6g)低于对照组( 1.2 0± 0 .2 4g,P=0 .0 5 ) ,CTX组瘤重 ( 0 .75± 0 .2 3 g)明显低于对照组 ( P<0 .0 5 )。免疫组化显示 ,Rg3组的微血管密度 ( 16.68± 8.0 0 )及血管内皮生长因子 ( VEGF)明显低于对照组 ( P<0 .0 5 )。结论　Objective To explore the inhibiting effect of ginsenoside Rg3 on the growth and angiogenesis of orthotopically xenotransplanted human breast infiltrating duct carcinoma in nude mice. Methods A total of 15 female nude mice having received xenotransplanted human breast infiltrating duct carcinoma were randomly divided into 3 groups. Ginsenoside Rg3 (5 mg/kg, qd), cyclophosphamid (26 mg/kg, qod) and control solution (0.5% sodium carboxymethyl cellulose, qd) were given to each group by gas-trogavage in 0.5 ml volume for 56 days. Breast cancer masses were collected for light microscope observation. The intra-tumoral microvessel density(MVD) and the expression of vascular endothelial growth factor(VEGF) were examined by immunohis-tochemical staining. Results The tumor weight of CTX group was significantly lower than that of control group (P<0.05), and the comparison between those of Rg3 group and control group was nearly reaching significant level. The MVD and VEGF of Rg3 group by immunohistochemical staining were significantly lower than those of control group (P<0.05). Conclusion Rg3 could inhibit the growth and angiogenesis of xenotransplanted human breast infiltrating duct carcinoma in nude mice.

关 键 词：人参皂甙RG3 乳腺浸润性导管癌 抗癌作用 微血管密度 血管内皮生长因子 

分 类 号：R737.9[医药卫生—肿瘤]