重组人生长激素对肾癌细胞株细胞周期动力学影响  

Effect of human growth hormone on cell cycle kinetics in GRC-1 cells line in vitro.

作　　者：周林玉[1] 汪健[2] 孙友文[1] 诸禹平[1] 许戈良[3] 柴仲培[3] 

机构地区：[1]合肥安徽省立医院泌尿外科,230001 [2]合肥安徽省立医院中心实验室,230001 [3]合肥安徽省立医院普外科,230001

出　　处：《安徽医学》2003年第4期4-7,共4页Anhui Medical Journal

摘　　要：目的　本实验试图阐明生长激素对于肾癌细胞有无促增殖和分化作用。方法　取对数生长期的人肾癌细胞株GRC -1,以氟尿嘧啶和 /或不同浓度的重组人生长激素 (rhGH)体外培养 ,2 4小时以后流式细胞仪测定细胞增殖周期和细胞凋亡等指标。结果　在空白对照组 ,G0 ～G1期的细胞数约占 5 3%,S期细胞数次之 ,G2 ～M期占不足 4%。在生长激素组 ,G0 ～G1期细胞数率降低 (P <0 .0 5 ) ,S期细胞百分率增高 (P <0 .0 1)。在单纯氟尿嘧啶组 ,G0 ～G1期细胞数增高 ,S期细胞数降低 ;同时加氟尿嘧啶和生长激素组与单纯氟尿嘧啶组相比 ,G0 ～G1期细胞数进一步增加 ,S期细胞数进一步降。结论　rhGH在体外作用于GRC -1细胞 ,能诱导细胞分化 ,促使静止细胞群进入增殖周期 ,并使处于DNA合成期的细胞增多 ,说明rhGH在体外有促进肾癌细胞生长增殖的作用 ,所以 ,我们推断肾癌细胞膜表面可能存在生长激素受体。氟尿嘧啶能使S期细胞数降低即抑制细胞的增殖 ,与氟尿嘧啶的作用机理相符。生长激素和氟尿嘧啶合用 ,S期细胞数进一步降低 ,说明生长激素能增强氟尿嘧啶抗肿瘤细胞生长增殖作用。Objectives To explore the effect of human growth hormone on cell cycle kinetics and apoptosis of human renal carcinoma cell line (GRC-1 cells).Methods GRC-1 cells were cultured for 24 hour in 1640 with or without Fluorouraci,but with rhGH at a concentration f 50,100,200 ng/ml respectively.Cells undergoing apoptosis and differentiation were determined by flow cytometry(FCM).Results rhGH had significant effect on GRC-1 cell cycle rate,percent G 0～G 1 phases dropped (P<0.05),percent S phase increased (P<0.01).addition of Fluorouraci to the culture medium could drop all rates of S phase.Addition of rhGH at different concentration and Fluorouraci to the culture medium could cell drop rates of S phase deeply.Conclusions GRC-1 cells might have expression for the GH receptor.In Vitor rhGH might induce differentiation of cells GRC-1 and promote cell cycle kinetics and strengthen Fluorouraci suppressing on percent S phase.

关 键 词：肾癌 细胞株 细胞周期动力学 重组人生长激素 细胞凋亡 

分 类 号：R737.11[医药卫生—肿瘤]