实时荧光定量PCR检测原发性肝癌中CDK4基因的表达  

Real-time fluorescence quantitative PCR in detecting CDK4 gene expression in hepatocellular carcinoma

作　　者：胡和平[1] 满晓波 王健 唐亮 王红阳 吴孟超[1] 邱秀华 

机构地区：[1]第二军医大学东方肝胆外科医院,上海200438 [2]东方肝胆外科研究所信号转导实验室 [3]上海安图医院普通外科

出　　处：《第二军医大学学报》2003年第7期773-775,共3页Academic Journal of Second Military Medical University

基　　金：上海市临床医学中心发展基金 ( ZX0 1B0 5 )

摘　　要：目的 :应用实时荧光定量 PCR法检测原发性肝细胞癌中 CDK 4基因的表达。 方法 :提取手术切除人肝癌和癌旁组织总 RNA并逆转录为 c DNA。应用实时荧光定量 PCR法观察人肝细胞癌组织及癌旁组织中 CDK 4的表达水平。结果 :2 0例肝细胞癌标本中有 18例肿瘤组织中 CDK 4基因表达明显高于癌旁肝组织 ,其中 7例高出 4倍以上。 结论 :实时荧光定量PCR可以准确定量测定基因的表达 ;CDK 4基因在肝细胞癌的发生。Objective:To quantitatively detect the expression level of CDK4 in primary hepatocellular carcinoma. Methods: The total RNA isolated from human HCC and adjacent liver tissue was reversely transcribed into cDNA. The real timefluorescence quantitative PCR method was used to analyze the expression level of CDK4 gene. Results: The real time fluorescence quantitative PCR method was performed successfully to precisely detect the mRNA level. The expression level of CDK4 gene was higher in tumor tissue than that in para tumor liver tissues in 18 cases of 20 cases of hepatocellular carcinoma (HCC). In 7 cases the expression level of CDK 4 showed 4 times difference between the CDK4 expression level of tumor tissue and that of para tumor liver tissue. Conclusion: The real time fluorescence quantitative PCR is the most precise method to quantitatively detect the mRNA level and can be used in gene expression changes. The CDK4 gene has higher expression in HCC tumor tissue cells than in para tumor liver tissue cells,indicating that it plays an important role in the development of the HCC.

关 键 词：原发性肝癌 实时荧光定量 PCR法 CDK4基因 基因表达 

分 类 号：R735.7[医药卫生—肿瘤]