植物基因工程疫苗高效表达载体的构建  

On Constructing Expressing Vectors for Genetic Engineering Vaccine

作　　者：李昌[1] 金宁一[1] 王罡[1] 张洪勇[1] 王萍[1] 季静[1] 郑敏[1] 

机构地区：[1]解放军军需大学植物基因工程研究中心,吉林长春130062

出　　处：《吉林农业大学学报》2003年第3期253-256,共4页Journal of Jilin Agricultural University

基　　金：863项目"(2001AA213071);"国家植物转基因中试及产业化基地"专项基金资助项目(J99-B-001)

摘　　要：分别将编码HIV-1的gag基因、gp120基因及gag-gp120嵌合基因,FMDV的P1全长基因和猪白细胞介素18(IL18)基因克隆到含CaMV35S启动子控制下的双元表达载体pBI121中,并通过冻融法导入农杆菌LBA4404,成功构建了能在植物中高效表达的5种植物表达载体:pBI121gag,pBI121gp,pBI121gg,pBI121P1,pBI121IL18。The main antigen gene encoded HIV-1, FMDV and IL18 were cloned into pBI121 vector including CaMV35S promoter, then transferred into Agrobacterium tumefaciens LBA4404 using the freezethaw method?Therefore five efficient expression vectors were successfully constructed It made a good foundation for producing new gene engineering vaccines using transgenic plants as bioreactors

关 键 词：基因工程疫苗 表达载体 抗原基因 转基因植物 农杆菌 植物生物反应器 

分 类 号：TQ464[化学工程—制药化工] TQ460.38