小麦新品系YW243抗条锈性鉴定和遗传分析  

Assessment and genetic analysis on the resistance of a new wheat line YW243 to stripe rust

作　　者：谢皓[1] 牛永春[2] 陈孝[1] 马有志[1] 吴立人[2] 林志珊[1] 

机构地区：[1]中国农业科学院作物育种栽培研究所 [2]中国农业科学院植物保护研究所,北京100094

出　　处：《植物病理学报》2003年第3期243-247,共5页Acta Phytopathologica Sinica

基　　金：国家"九五"科技攻关项目 (96 C0 1 0 1 0 6 0 9) ;北京市自然科学基金项目 (592 0 0 8)

摘　　要：YW2 4 3是最近培育出的兼抗条锈病、白粉病和黄矮病的小麦新品系 ,本文对其条锈病抗性进行研究。小麦条锈菌苗期接种鉴定表明 ,YW2 4 3高抗CY2 9、CY30、CY31、CY32、CYSu 11等我国条锈菌流行小种。用 2 6个来自世界各地的菌系接种进行基因推导 ,结果表明 ,YW2 4 3具有较宽的抗谱 ,试验中 2 1个已知基因系 ,仅有Yr5的抗性与YW2 4 3相似 ,但YW2 4 3的系谱表明不含有Yr5基因 ,因此YW2 4 3很可能含有一个新的抗条锈病基因。用YW2 4 3和京 771分别作为抗感亲本进行杂交 ,后代分离结果表明 ,YW2 4 3对条锈菌CY31的抗性由 1个显性基因控制。分子标记初步研究表明 ,该基因与RAPD引物OPY0 8扩增出的YW243, a recently developed new source of resistance to wheat stripe rust, powdery mildew and BYDV, was studied on its resistance to stripe rust. The results of seedling test showed that it was resistant to all the contemporary prevalent races of Puccinia striiformis in China, including CY29, CY30, CY31, CY32 and CYSu 11. The result of postulation study showed that it had a wide resistance spectrum, with resistance to all of the 26 isolates (races) collected from various regions of the world. Of the 21 lines with known Yr genes, only the line with Yr5 had an RS similar to that of YW243, but the pedigree of YW243 showed that the progenitor of YW243 did not include Yr5 , indicating that the resistance gene of YW243 is likely a new stripe rust resistance gene. A genetic study of a cross using YW243 and Jing 771 as resistant and susceptible parents revealed that the stripe resistance of YW243 to CY31 was controlled by a single dominant gene. A preliminary molecular marker study revealed that this gene was linked to a specific DNA segment amplified by a RAPD primer OPY08.

关 键 词：小麦 新品系 YW243抗条锈性 鉴定技术 遗传分析 

分 类 号：S512.1[农业科学—作物学]