SO_2衍生物诱发蚕豆根尖细胞微核和后期异常的研究  

MICRONUCLEUS AND ANAPHASE ABERRATIONS IN VICIA FABA ROOT TIPS INDUCED BY THE DERIVATIVES OF SULFUR DIOXIDE

作　　者：仪慧兰[1] 李新锋[1] 孟紫强[1] 

机构地区：[1]山西大学环境医学与毒理学研究所,生命科学与技术学院太原030006

出　　处：《植物研究》2003年第3期308-311,共4页Bulletin of Botanical Research

基　　金：山西省自然科学基金和山西省教委环境科学重点学科基金资助 (KD0 0-2 6)。

摘　　要：研究SO2 体内衍生物———亚硫酸钠和亚硫酸氢钠混合液 ( 3:1mmol·L- 1 /mmol·L- 1 )诱发蚕豆根尖细胞微核和后期异常的效应。结果表明 :SO2 衍生物处理可诱发蚕豆根尖间期细胞微核和核芽 ,使分裂后期出现多种染色体异常 ,如断片、桥以及滞后染色体等。异常细胞中以微核细胞和染色体断裂细胞居多。在一定浓度范围内 ,细胞异常率与处理液浓度之间表现正的线性相关。这些研究结果表明 ,蚕豆根尖间期微核和后期染色体异常有可能用作检测SO2 污染的生物剂量计。The micronucleus and anaphase aberrations in Vicia faba root tips induced by a mixture of sodium sulfite and sodium bisulfite 3:1, a solution form of sulfur dioxide, were investigated. The results showed that sodium bisulfite solution caused micronucleus and nuclear buds in interphase cells and also anaphase aberrations such as chromosomal segment, bridge and lagging in division cells. The abnormal cells with micronuclei or chromosomal breakage were the most of all aberrant cells in the Vicia root tips after exposure to bisulfite. The frequencies of cells with various aberrations had positive response to the treated concentrations. These results indicate that SO 2 can induce cytogenetic damage in plant cells and both frequencies of Vicia micronucleus and anaphase aberrations could be used as the biological dosimeters to monitor SO 2 pollutant in the environment.

关 键 词：SO2衍生物 诱发 蚕豆 根尖细胞 微核 后期异常 生物剂量计 染色体异常 

分 类 号：S643.6[农业科学—蔬菜学] Q943[农业科学—园艺学]