小鼠体外成熟与激素超排卵母细胞核型分析  

COMPARISON OF MOUSE OOCYTE KARYOTYPES BETWEEN HORMONE INDUCTION OVULATION AND CULTURE IN VITRO MATURATION

作　　者：邝素华[1] 龚春柳[1] 吴锐辉[1] 陈仲贤[1] 胡一骏[1] 曾如辉[1] 谢庆东[1] 黄天华[1] 

机构地区：[1]汕头大学医学院生殖医学研究中心,广东汕头515031

出　　处：《癌变．畸变．突变》2003年第3期133-137,F003,共6页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金资助(No:30170481)

摘　　要：目的 :评价激素超排(HIO)和体外培养成熟(CVM)两种方法对小鼠卵母细胞染色体的影响。 方法 :通过HIO与CVM分别获得100个、80个成熟小鼠卵母细胞 ,制备染色体标本进行核型分析。 结果 :在HIO组 ,超单倍体率、非整倍体率、染色体结构畸变率、结构畸变卵母细胞率、退化卵母细胞率和染色体分离均数依次为2.0 %、4.0 %、2.0 %、2.0 %、8.0 %和0.13 ;在CVM组 ,上述实验参数依次为4.0 %、8.0 %、7.5 %、6.3 %、12.5 %和0.325;两组所有实验参数经统计学处理没有显著差异。 结论 :经体外培养成熟的与经激素超排的小鼠卵母细胞其质量和染色体核型没有本质上的差异。将此结果外推到人类 ,提示未成熟卵母细胞经体外培养成熟在人类辅助生殖领域具有广泛的应用前景。Purpose: This study was designed to evaluate the effects of two methods, hormone induction ovulation(HIO) and culture in vitro maturation (CVM), on the quality and chromosomal complements in mouse oocytes. Methods: 100 matured mouse oocytes were obtained by HIO and 80 by CVM. Their chromosomal complements were prepared and analyzed. Results: The hyper haploid rate, aneuploid rate, chromosomal structurally aberration rate, structurally aberrated oocyte rate, degenerative oocyte rate and mean number of splitting chromosomes in HIO group were 2.0 %,4.0 %,2.0 %, 2.0 %, 8.0 % and 0.13 subsequently, and were 4.0 %, 8.0 %, 7.5 %, 6.3 %, 12.5 % and 0.32 5 in CVM group. All the parameters between two groups are not signifi cantly different. Conclusion: The qualities and chromosomal complements of mouse oocytes obtained by CVM were not naturally different from those by HIO. Extrapolating to human, this result suggested that the maturation in vitro of immature oocyte would have great potential for human assisted reproduction.

关 键 词：卵母细胞 体外培养成熟 激素超排 染色体 

分 类 号：R394.2[医药卫生—医学遗传学]