类黄酮3′,5′羟基化酶基因的克隆及转化矮牵牛  

Cloning of Flavonoid 3', 5'-Hydroxylase Gene and Transformation into Petunia hybrida

作　　者：张林玲[1] 金志强[1] 苏伟[1] 徐碧玉[1] 

机构地区：[1]中国热带农业科学院热带作物生物技术国家重点实验室,海口571101

出　　处：《科学技术与工程》2003年第4期352-355,共4页Science Technology and Engineering

摘　　要：类黄酮3’,5’羟基化酶(Flavonoid 3’,5’-hydroxylase,F3＇,5＇H)是花色素苷代谢途径中的一个关键酶,它使花色素的合成方向趋向于形成蓝色色素翠雀素。利用PCR方法,从“紫蓝色”品系的矮牵牛(Petunia hybrida)总DNA中,克隆到编码F3’,5＇H的Hf1、Hf2基因。将Hf2基因正向插入到植物表达中间载体中,通过土壤农杆菌介导的方法转化“淡粉色”品系的矮牵牛。现已得到PCR阳性植株。Flavonoid 3',5'-hydroxylase(F3',5'H) is the key enzyme in the biosynthesis of anthocyanins, which willdivert it towards the production of delphinidin pigments. Hf1 and Hf2 genes encoding F3',5'H were isolated from Petu-nia hybrida of the purple flower with PCR. Also a construct of Hf2 gene in a sense-orientation was constructed and thentransformed it to Petunia hybrida of the light pink flower mediated by Agrobacterium tumefaciens EHA105. The trans-formed plants are identified by PCR.

关 键 词：类黄酮3′ 5′羟基化酶基因 基因克隆 矮牵牛 转基因花卉 花色素苷 植物基因工程 

分 类 号：Q943.2[生物学—植物学]