小剂量3-硝基丙酸预处理后鼠脑海马脑源性促红细胞生成素的表达  

Expression of brain-derived erythropoietin in the hippocampus of low dose 3-NPA preconditioned gerbils

作　　者：朱红灿[1] 孙圣刚[1] 李红戈[1] 高晓群[2] 

机构地区：[1]华中科技大学同济医学院附属协和医院神经内科,武汉430022 [2]华中科技大学同济医学院神经生物学系,武汉430030

出　　处：《郑州大学学报（医学版）》2003年第4期515-518,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：教育部留学回国人员基金项目 2 0 0 1 3 45

摘　　要：目的 :探讨小剂量 3 硝基丙酸 (3 nitropropionicacid ,3 NPA)预处理后鼠脑海马脑源性促红细胞生成素(Epo)的表达变化 ,揭示 3 NPA预处理的机制。方法 :雄性沙土鼠 4 8只 ,随机分为正常对照组 (n =12 )和 3 NPA预处理组 (n =36 )。 3 NPA预处理组腹腔一次性注射 3 NPA 5mg/kg后 1d、3d、5d ,应用免疫组织化学和免疫印迹法观察海马脑源性Epo的表达变化。结果 :腹腔注射 3 NPA后 1d、3d ,海马区可检测到脑源性Epo的表达 ,腹腔注射 3 NPA后 5d ,海马区Epo的表达极低 ,正常对照组无Epo表达。结论 :脑源性Epo的表达变化和 3 NPA预处理诱导脑缺血耐受的时间相一致 ,提示Epo的表达与小剂量 3Aim: To explore the expression of brain derived erythropoietin in the hippocampus of low dose 3 nitropropionic acid(3 NPA) preconditioned gerbils and the mechanism of 3 NPA preconditioning. Methods:Gerbils were pretreated with 3 NPA at the dose 5 mg/kg intraperitoneally.At 1 d, 3 d, and 5 d after pretreatment, expression of brain derived erythropoietin was detected by immunohistochemistry and Western blot.Results:After 1d and 3 d after pretreatment, brain derived erythropoietin showed up in hippocampus. Expression of erythropoietin was detected at 5 d after pretreatment.Conclusion: The time between expression of brain derived erythropoietin and ischemic tolerance induced by low dose 3 NPA is matched, suggesting expression of brain derived erythropoietin might be involved in ischemic tolerance induced by low dose 3 NPA.

关 键 词：小剂量 3-硝基丙酸 预处理 脑海马 脑源性促红细胞生成素 表达 

分 类 号：R363.22[医药卫生—病理学]