Role of inducible nitric oxide synthase expression in aberrant crypt foci-adenoma-carcinoma sequence  

作　　者：Mei-Hua Xu Chang-Sheng Deng You-Qing Zhu Jun Lin Department of Gastroenterology,Zhongnan Hospital,Wuhan University,Wuhan 430071,Hubei Province China 

出　　处：《World Journal of Gastroenterology》2003年第6期1246-1250,共5页世界胃肠病学杂志（英文版）

摘　　要：AIM: To investigate the expression of inducible nitric oxidesynthase (iNOS) in aberrant crypt foci (ACF) -adenomacarcinoma sequence and its relation with tumor cellapoptosis, proliferation and angiogenesis.METHODS: The expression of iNOS, proliferating cellnuclear antigen (PCNA) and microvessel density (MVD) indifferent stages of colorectal cancer were studied by immunohistochemical method from 30 normal tissues, 30nonhyperplastic ACF, 30 hyperplastic ACF, 30 dysplastic ACF,30 adenomas and 60 carcinomas. The apoptotic cells were detected by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) method using an Apop Tag in situ detection kit.RESULTS: The immunoreactivity of iNOS significantly increased in the transition from hyperplastic ACF to dysplastic ACF. This transition was associated with a significant decrease in the apoptotic index (AI) (0.73+0.37 vs0.61±0.35, P＜0.05)and significant increases in the PCNA labeling index (LI)(27.3+2.80 vs 40.3+3.11, P＜0.01) and microvessel density (MVD) (55±11.5 vs 70±13.2, P＜0.01). The expression of iNOS was in low levels and positively correlated with PCNALI (r=0.812, P＜0.01) and MVD (r=0.863, P＜0.01) during transition from normal mucosa to nonhyperplastic ACF and hyperplastic ACF. The expression of iNOS was in high levels and positively correlated with AI (r=0.901, P＜0.01) after transition from hyperplastic ACF to dysplastic ACF, adenoma and carcinoma.CONCLUSION: The results suggest that the transition from hyperplastic ACF to dysplastic ACF may be a crucial step in the ACF-adenoma-carcinoma sequence, in which iNOS plays an important role by regulating tumor cell apoptosis,proliferation and angiogenesis.AIM:To investigate the expression of inducible nitric oxide synthase (iNOS) in aberrant crypt foci (ACF) -adenoma- carcinoma sequence and its relation with tumor cell apoptosis,proliferation and angiogenesis. METHODS:The expression of iNOS,proliferating cell nuclear antigen (PCNA) and microvessel density (MVD) in different stages of colorectal cancer were studied by immunohistochemical method from 30 normal tissues,30 nonhyperplastic ACF,30 hyperplastic ACF,30 dysplastic ACF, 30 adenomas and 60 carcinomas.The apoptotic cells were detected by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) method using an Apop Tag in situ detection kit. RESULTS:The immunoreactivity of iNOS significantly increased in the transition from hyperplastic ACF to dysplastic ACF.This transition was associated with a significant decrease in the apoptotic index (AI)(0.73±0.37 vs 0.61±0.35,P<0.05) and significant increases in the PCNA labeling index (LI) (27.3±2.80 vs 40.3±3.11,P<0.01) and microvessel density (MVD)(55±11.5 vs 70±13.2,P<0.01).The expression of iNOS was in low levels and positively correlated with PCNA- LI (r=-0.812,P<0.01) and MVD (r=0.863,P<0.01) during transition from normal mucosa to nonhyperplastic ACF and hyperplastic ACE The expression of iNOS was in high levels and positively correlated with AI (r=0.901,P<0.01) after transition from hyperplastic ACF to dysplastic ACF,adenoma and carcinoma. CONCLUSION:The results suggest that the transition from hyperplastic ACF to dysplastic ACF may be a crucial step in the ACF-adenoma-carcinoma sequence,in which iNOS plays an important role by regulating tumor cell apoptosis, proliferation and angiogenesis.

关 键 词：一氧化氮合酶 肿瘤发生学 增殖细胞核抗原 微血管密度 

分 类 号：R730.2[医药卫生—肿瘤]