益肾降浊汤对脑缺血再灌注大鼠海马EAA含量的影响  

Effect of Yishen Jiangzhuo Decoction on the EAA Content of Hippocampus in Cerebral Ischemia Reperfusion Rats

作　　者：高维娟[1] 钱涛[2] 丛斌[3] 

机构地区：[1]河北省承德医学院,067000 [2]河北省承德医学院附属医院 [3]河北医科大学法医学教研室

出　　处：《中西医结合心脑血管病杂志》2003年第6期319-321,共3页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：河北省科技厅资助项目 (No:982 761 87D)

摘　　要：目的 :观察益肾降浊汤对脑缺血再灌注大鼠海马组织EAA含量的影响。方法 :用氨基酸自动分析仪测定游离氨基酸浓度。结果 :第 1天模型大鼠海马Glu、Asp含量分别是 ( 8.2 6± 2 .13 ) μmol/g、( 2 .10± 0 .93 ) μmol/g ,第 7天含量分别是 ( 7.2 7± 2 .2 0 ) μmol/g、( 1.87± 2 .0 1) μmol/g ,第 15天含量分别是 ( 9.3 6± 2 .2 4) μmol/g、( 1.42± 1.2 1) μmol/g ,均较正常对照组显著降低 (P <0 .0 5～ 0 .0 1)。而中药组大鼠第 1天脑海马Glu、Asp含量分别是 ( 9.90± 3 .2 0 )μmol/g、( 2 .95± 1.73 ) μmol/g ,第 7天含量分别是 ( 10 .49± 2 .3 4)μmol/g、( 3 .0 3± 1.0 9) μmol/g ,第15天含量分别是 ( 11.0 5± 2 .5 4)μmol/g、( 3 .0 5± 1.41) μmol/g ,均高于同期模型组 (P <0 .0 5 )。结论 :益肾降浊汤可提高缺血再灌注后脑组织细胞内的EAA含量 ,调节突出间隙EAA浓度 ,对缺血神经元有保护作用。Objective:To observe the effect of Yishen Jiangzhuo decoction on the EAA content of hippocampus in cerebral ischemia reperfusion rats.Methods:The EAA content was measured by Amino-acid auto-analyzer.Results:The contents of Glu and Asp in hippocampus were respectively(8.26±2.13)μmol/g、(2.10±0.93)μmol/g on the 1st day and (7.27±2.20)μmol/g、(1.87±2.01)μmol/g on the 7th day and (9.36±2.24)μmol/g、(1.42±1.21)μmol/g on the 15th day in the model group, which significantly decreased compared with the control group (P<0.05～0.01). While in the decoction group the Glu and Asp contents of hippocampus were respectively (9.90±3.20)μmol/g、(2.95±1.73)μmol/g on the 1st day and (10.49±2.34)μmol/g、(3.03±1.09)μmol/g on the 7th day and (11.05±2.54)μmol/g、(3.05±1.41)μmol/g on the 15th day, were higher than the model group on the same experimental day (P<0.05).Conclusion:Yishen Jiangzhuo decoction might increase the EAA content of hippocampus in cerebral ischemia reperfusion rats, regulate the EAA density in synaptic cleft and preserve neurons.

关 键 词：脑缺血 再灌注损伤 益肾降浊汤 大鼠 海马 EAA 治疗 游离氨基酸 

分 类 号：R285.5[医药卫生—中药学]