RP-HPLC法测定人体中富马酸喹硫平血药浓度  

Determination of quetiapine fumarate serum concentration with PR - HPLC.

作　　者：林治光[1] 翁毅仁[1] 庄冬梅[1] 蒋继承[1] 李华芳[1] 顾牛范[1] 

机构地区：[1]上海市精神卫生中心生化药理室,200030

出　　处：《四川精神卫生》2003年第3期140-142,共3页Sichuan Mental Health

摘　　要：目的 建立测定富马酸喹硫平血药浓度的方法。方法 应用反相高效液相色谱技术,YWG-C_(18)(150mm×4.6mm,10μm)色谱柱,以氯氮为内标,乙醚为萃取剂,甲醇-水-四甲基乙二胺(63:45:0.3)为流动相,冰乙酸调节pH至5.80,流速1mL·min^(-1),检测波长250nm,柱温40℃。结果 富马酸喹硫平及内标与血清杂质可完全分离,保留时间分别为4.6min和6.1min,在10～2400ng·mL^(-1)浓度范围内呈良好的线性关系(r=0.9998),平均回收率98.2％～101.1％,日内、日间RSD在2.7％～6.9％之间,最低检测浓度为5ng·mL^(-1)。结论 该方法操作简便,结果准确,专属性强,具有一定的实用价值。Objective To establish a RP-HPLC method for determination of quetiapine fumarate serum concentration. Methods Instruments, reagents, and conditions adopted in the analysis were: A YWG - C18 column (150mm×4.6 mm,10μm), chlordiazepoxide was used as internal standard, the detective wavelength was 250nm,the mobile phase of MeOH: H2O: TEMDA =63: 45: 0.3 ,its pH was adjusted to 5. 80 with glacial acetic acid, the flow rate was 1.0 mL·min-1 and the column temperature was 40℃. Results Quetiapine fumarate and internal standard can be completely separated with a liner range of 10 - 2400ng · mL-1 ,and correlation coefficients was 0. 9998. The lowest detective concentration was 5 ng · mL-1. The average recovery was 98. 2% - 101. 1% with RSD of intra - day and inter - day assay were within 2.7% -6.9%. Conclusion A simple , accurate, selective and suitable method for determination of quetiapine fumarate serum concentration was established.

关 键 词：富马酸喹硫平 血药浓度 HPLC 

分 类 号：R749.053[医药卫生—神经病学与精神病学]