Immunoinhibitory effect of soluble HLA-G1 on the cytotoxicity of NK92 cells  

作　　者：TIAN Xinghui, LI Zhiwei, LIU Yabing, WANG Yun, TANG Haiyang and FENG Meifu(The National Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China) 

出　　处：《Progress in Natural Science:Materials International》2003年第3期191-195,共5页自然科学进展·国际材料（英文版）

基　　金：Supported by the National Key Laboratory of Biomembrane and Membrane Biotechnology

摘　　要：HLA-G (human leukocyte antigen-G) is a non-classical HLA class Ⅰ molecule, playing an important immuno-modulatory role in maintaining maternal immune tolerance of the semiallogenic fetus and organ transplantation. In this study, the cDNA sequence of extracellular domain of HLA-G1 was subcloned into the pET28a vector and a soluble 35 kD fusion protein (His-sHLA-G1) with six histine residues was obtained. In the 4 h 51Cr-release assay the fusion protein obviously inhibited the cytotoxicity of NK92 cells in a dose-depen-dent manner. These results indicated that sHLA-G1, as an activated immunoinhibitor, may provide an effective approach to overcoming the immune rejection of transplantation.HLA-G (human leukocyte antigen-G) is a non-classical HLA class I molecule, playing an important immuno-modulatory role in maintaining maternal immune tolerance of the semiallogenic fetus and organ transplantation. In this study, the cDNA sequence of extracellular domain of HLA-G1 was subcloned into the pET28a vector and a soluble 35 kD fusion protein (His-sHLA-G1) with six histine residues was obtained. In the 4 h 51Cr-release assay the fusion protein obviously inhibited the cytotoxicity of NK92 cells in a dose-dependent manner. These results indicated that sHLA-G1, as an activated immunoinhibitor, may provide an effective approach to overcoming the immune rejection of transplantation.

关 键 词：sHLA-G1 NK92 CYTOTOXICITY immunoinhibition. 

分 类 号：R392.1[医药卫生—免疫学]