青蒿素诱导K562细胞凋亡研究  

Apoptosis Induced by Artemisinin in K562 Cells

作　　者：董海鹰[1] 王知非[2] 宋维华[1] 王玲[1] 杨宝峰[1] 

机构地区：[1]哈尔滨医科大学药理教研室,黑龙江哈尔滨150086 [2]哈尔滨医科大学第三临床医院,黑龙江哈尔滨150086

出　　处：《中国肿瘤》2003年第8期473-475,共3页China Cancer

基　　金：国家自然科学基金(30271599)资助

摘　　要：[目的]研究青蒿素对体外培养的K562细胞的凋亡诱导作用及机制。[方法]MTT法测定药物对K562细胞生长的抑制作用;透射电镜观察药物对K562细胞形态学的影响;流式细胞仪检测经药物作用后的细胞凋亡率;用Rhodamine(Rh123)染色法检测细胞线粒体跨膜电位(Δψm)的变化。[结果]青蒿素对K562细胞生长有明显的抑制作用,细胞经药物作用48小时后的IC50为26.5μmol/L;电镜观察细胞有典型的凋亡形态特征;细胞凋亡率在一定范围内与药物浓度正相关。给药后跨膜电位明显下降。[结论]青蒿素可抑制K562细胞的生长,诱导K562细胞跨膜电位下降而导致细胞凋亡。To investigate the effect of artemisinin(ART)on the induced apoptosis in K562cells and its mechanism in vitro.The inhibition of ART on K562cells were evaluated by means of MTT assay.The morphological changes of ART on K562cells were observed under transmission electron-microscope.Cell apoptosis rate influenced by ART was assessed by flow cytometer.Mitochondrial trans-membrane potential was assessed by dyeing of the Rhodamine(Rh123).The growth of K562cells was inhibited after being acted by ART for48hours in vitro,the IC 50 of ART was26.53μmol/L.Some typically early and final phase changes of apoptosis were revealod under transmission electromicroscope.Apoptosis rate was associated with ART concentration.Transmembrane potential was significantly de-creased after ATR was administered.[Conclusion]ART can inhibit the proliferation of K562cells and in-duce apoptosis,which closely associated with the decreasing of transmembrane potential.

关 键 词：青蒿素 K562细胞 MTT法 凋亡 

分 类 号：R73－3[医药卫生—肿瘤]