L-精氨酸、L-硝基精氨酸对红藻氨酸诱导大鼠癫痫及其海马结构中iNOS mRNA表达的影响  

Effect of L-arginine or L-nitroarginine on kainic acid induced seizure and expression of iNOS mRNA in the rats' hippocampus

作　　者：孙艺平[1] 孙长凯[1] 范明[2] 赵杰[1] 韩大跃[3] 王吉庆[3] 王冬梅[4] 彭岩[4] 宫德正[4] 傅雷[4] 戴淑芳[4] 徐红[4] 

机构地区：[1]大连医科大学脑疾病研究所,大连116027 [2]军事医学科学院基础医学研究所神经生物研究室,北京100850 [3]解放军210医院神经科,大连116021 [4]大连医科大学机能实验室,大连116027

出　　处：《中国药理学通报》2003年第8期889-891,共3页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助 (No 3954 0 0 0 2 30 0 70 2 6 7);辽宁省教育厅高校科研项目基金资助 (No 2 0 2 2 0 32 4 6 )

摘　　要：目的　观察诱导型一氧化氮合酶 (iNOS)在红藻氨酸(KA)癫痫大鼠海马内的表达及L 精氨酸 (L Arg)和L 硝基精氨酸 (L NNA)慢性干预的影响。方法　采用惊厥剂量的KA(1 0mg·kg- 1 )诱导大鼠癫痫发作 ,以NOS抑制剂L NNA(50mg·kg- 1 )和NO前体L Arg(40mg·kg- 1 )进行干预 ,对大鼠的癫痫发作行为及KA后不同时间点的海马内i NOSmRNA ,通过RT PCR观察其表达。结果　KA可使动物发生时间相关性癫痫发作 ,L NNA预处理后使KA诱导的癫痫发作明显加重 ,而L Arg预处理后使KA诱导的癫痫发作减弱。iNOSmRNA在KA处理后 3h开始有微弱的表达 ,且随着时间的延长逐渐增加 ,2 4h达到最高水平 ,2d及3d时未见表达 ,但 7d时又出现高表达 ;经L NNA预处理的动物 ,KA后 1h其海马结构中未出现iNOSmRNA ,但L Arg预处理后再给予KA后 1h ,可见微弱的iNOSmRNA表达。结论　红藻氨酸给药后一定时间 ,癫痫大鼠海马结构中可出现iNOSmRNA表达 ,LAIM To observe the expression of inducible nitric ox ide synthase (iNOS) in the rats' hippocampus of kainic acid (KA) induced-seizur es and the effect of L-arginine(L-Arg) or L-nitroarginine(L- NNA) chronic intervention before KA. METHODS The expression of iN OS mRNA by RT-PCR and behaviour were observed after administration of convulsan t dose of KA (10 mg·kg -1 ) and pretreatment with NO predecessor (L-Ar g, 40 mg·kg -1 ) or a inhibitor of NOS(L-NNA, 50 mg·kg -1 ) befo re KA. RESULTS The time-dependent seizures were induced on rats after giving KA, and were improved and serious in the animals with L-NNA pr etreatment, but they were alleviated by L-Arg pretreatment before KA. Compa red with control, iNOS mRNA expression was feebly at KA 3 h, continuative improv ement accompanying KA time prolonged, and it was markedly enhanced at KA 24 h. I t couldn't be examined at KA 2 d or 3 d, and was obviously improved at KA 7 d a gain. iNOS mRNA appeared weak in the rats hippocampus with L-Arg pretreatme nt after KA 1 h, whereas it wasn't found in L-NNA pretreatment animals. CONCLUSION iNOS mRNA expression appeared in the hippocampus of seizu re rats at a few time after KA, and L-Arg chronic intervention before KA ha s a little effect on it.

关 键 词：诱导型-氧化氮合酶 组成型一氧化氮合酶 L-硝基精氨酸 L-精氨酸 红藻氨酸 癫痫 

分 类 号：R－332[医药卫生] R282.71