纯钛表面吸附硫酸软骨素A对成骨细胞生长的影响  

The effect of chondroitin sulfate - A attached titanium surface on the culture of osteoblast

作　　者：樊洪[1] 陈治清[2] 杨连举[1] 魏大鹏[2] 

机构地区：[1]天津市口腔医院,300041 [2]四川大学华西口腔医学院

出　　处：《现代口腔医学杂志》2003年第4期288-291,共4页Journal of Modern Stomatology

摘　　要：目的 在体外研究纯钛表面吸附硫酸软骨素 A(Chondroitin Sulfate-A,CS-A)后对成骨细胞生物学行为的影响。方法 在吸附不同浓度的CS—A钙处理钛片表面培养成骨细胞,检测其生长情况、碱性磷酸酶活性及细胞层钙含量。结果 钙处理钛片在吸附 CS—A后促进了成骨细胞的增殖,增加了成骨细胞层钙聚集,促进成骨细胞的矿化,同时还增高了成骨细胞的碱性磷酸酶活性,促进了细胞的分化。结论 钛吸附GS-A后提高了其生物相容性。Objective To elucidate the effect of Chondroitin Sulfate - A(CS- A)attached titanium surface on the culture of osteoblast in vitro. Methods Osteoblast was cultured on the Titanium surfaces that had been attached various concentrations of CS - A, cell proliferation quantity, calcium content and alkaline phosphatase activity of the cell layers were examined. Results Titanium surface attached various concentrations of CS- A can promote the proliferation of osteoblast, calcium accumulation in the cell layers and alkaline phosphatase activity. Conclusion Titanium surfaces attached CS- A improve their biocompatibility.

关 键 词：纯钛表面 吸附 硫酸软骨素A 成骨细胞生长 生物相容性 体外研究 蛋白质 含量测定 碱性磷酸酶 

分 类 号：R780.2[医药卫生—口腔医学]