罗红霉素与紫色素的荷移反应及其测定  

Spectrophotometric Determination of Roxithromycin Based on the Charge Transfer Reaction between Roxithromycin and Purpurin

作　　者：李华侃[1] 吕欣[1] 赵桂芝[1] 赵延清[1] 

机构地区：[1]锦州医学院化学教研室,锦州121001

出　　处：《分析化学》2003年第7期833-835,共3页Chinese Journal of Analytical Chemistry

摘　　要：建立了一种测定罗红霉素的方便而快捷的荷移分光光度法。罗红霉素与紫色素在乙醇介质中发生电荷转移反应 ,荷移络合物在 5 4 4nm处有最大吸收 ;表观摩尔吸光系数是 6 .5 6× 10 3L·mol- 1 ·cm- 1 ,该络合物的组成是 1∶1,稳定常数是 3× 10 4 。药物浓度在 0～ 12 0mg L范围内服从比耳定律。当罗红霉素浓度为 5 0mg L时 ,5次测定结果的相对标准偏差为 1.3%。利用本法测定了罗红霉素胶囊中有效成分的含量 ,并与文献法进行比较 ,二者结果基本吻合 ,回收率在 96 %以上。A spectrophotometric method for the determination of roxithromycin has been developed based on the charge transfer reaction between roxithromycin as donor and purpurin as acceptor. The Composition ratio and stability constant of charge transfer complex are 1 : 1 and 3 x 10(4) respectively. The apparent molar absorptivity of complex at 544 nm is 6. 56 x 10(3) L . mol(-1) . cm(-1). Beer's law is obeyed in the range of 0 similar to 120 mg/L of roxithromycin. The relative standard deviation of five determinations is 1. 3 %. The proposed method has been applied to the determination of roxithromycin in capsules with satisfactory results.

关 键 词：罗红霉素 紫色素 电荷转移反应 测定 分光光度法 大环内酯类抗生素 

分 类 号：TQ465[化学工程—制药化工]