固定化基因工程菌的培养和hEGF表达  

IMMOBILIZED CULTIVATION OF RECOMBINANT E. coli JM101 AND hEGF EXPRESSION

作　　者：陈新爱[1] 徐志南[1] 沈文和[1] 岑沛霖[1] 

机构地区：[1]浙江大学化学工程与生物工程学系生物工程研究所,浙江杭州310027

出　　处：《化工学报》2003年第7期1021-1024,共4页CIESC Journal

摘　　要：A genetic recombinant hEGF producer E.coli JM101 was immobilized in polyurethane foam matrix and a three-phase fluidized-bed bioreactor was used to produce human epidermal growth factor (hEGF).The results indicated that polyurethane foam is an adequate immobilization material. With the addition of polyurethane foam matrix, the free cell density, plasmid stability and hEGF productivity were higher than those without the foam matrix, respectively. The two-stage culture of the immobilized cells was further carried out, in which, the first stage was performed in a three-phase fluidized-bed bioreactor for cell growth and the second stage was performed in bubbling bed for product expression. At a dilution rate of 0.2 h -1 , after continuous cultivation for more than 60 h, the free cell density and product expression were maintained relatively stable. The hEGF productivity was still higher than 140 mg·L -1 and the fraction of plasmid-carrying was more than 27%. The hEGF productivity in two-stage process was higher than the batch process.A genetic recombinant hEGF producer E.coli JM101 was immobilized in polyurethane foam matrix and a three-phase fluidized-bed bioreactor was used to produce human epidermal growth factor (hEGF).The results indicated that polyurethane foam is an adequate immobilization material. With the addition of polyurethane foam matrix, the free cell density, plasmid stability and hEGF productivity were higher than those without the foam matrix, respectively. The two-stage culture of the immobilized cells was further carried out, in which, the first stage was performed in a three-phase fluidized-bed bioreactor for cell growth and the second stage was performed in bubbling bed for product expression. At a dilution rate of 0.2 h -1 , after continuous cultivation for more than 60 h, the free cell density and product expression were maintained relatively stable. The hEGF productivity was still higher than 140 mg·L -1 and the fraction of plasmid-carrying was more than 27%. The hEGF productivity in two-stage process was higher than the batch process.

关 键 词：聚氨酯泡沫 hEGF重组菌 固定化培养 质粒稳定性 

分 类 号：Q936[生物学—微生物学]