拟低共熔体系中脂肪酶水解拆分萘普生酯  

LIPASE-CATALYZED RESOLUTION OF METHYL 2-(6-METHOXY-2-NAPHTHYL)PROPIONATE IN PSEUDO-EUTECTIC SYSTEMS

作　　者：鞠培培[1] 黄克文[1] 曹建军[1] 沈树宝[1] 胡永红[1] 欧阳平凯[1] 

机构地区：[1]南京工业大学制药与生命科学学院,江苏南京210009

出　　处：《化工学报》2003年第7期1025-1028,共4页CIESC Journal

基　　金：国家自然科学基金 (No 2 99760 19;2 0 1760 18);江苏省自然科学基金 (No BK9912 3 )~~

摘　　要：In this paper, methyl 2-(6-methoxy-2naphthyl)propionate(naproxen)was asymmetrically hydrolyzed by candida rugosa lipase in pseudo-eutectic and organic solvent systems. The result showed that S -(+)-naproxen with enantiomeric excess more than 95% could be obtained with commercially available candida rugosa lipase in both systems. In organic solvent system, 24.1% reaction conversion of naproxen methylester could be obtained after 96 hours, while in pseudo-eutectic systems, the conversion was 40.1% after 72 h. Furthermore,only one fifth organic solvent volume was used in pseudo-eutectic systems.Compared to organic solvent systems, the effects of lipase concentration, substrate and water concentration, and several different kinds of adjuncts on reaction conversion were studied in pseudo-eutectic system. Also,under the optimium pseudo-eutectic enzymatic reaction conditions, the reaction conversion was 45.8%.In this paper, methyl 2-(6-methoxy-2naphthyl)propionate(naproxen)was asymmetrically hydrolyzed by candida rugosa lipase in pseudo-eutectic and organic solvent systems. The result showed that S -(+)-naproxen with enantiomeric excess more than 95% could be obtained with commercially available candida rugosa lipase in both systems. In organic solvent system, 24.1% reaction conversion of naproxen methylester could be obtained after 96 hours, while in pseudo-eutectic systems, the conversion was 40.1% after 72 h. Furthermore,only one fifth organic solvent volume was used in pseudo-eutectic systems.Compared to organic solvent systems, the effects of lipase concentration, substrate and water concentration, and several different kinds of adjuncts on reaction conversion were studied in pseudo-eutectic system. Also,under the optimium pseudo-eutectic enzymatic reaction conditions, the reaction conversion was 45.8%.

关 键 词：拟低共熔体系 脂肪酶 萘普生 水解拆分 

分 类 号：Q814[生物学—生物工程]