有机溶剂性质及其水含量对酶催化合成含D-氨基酸残基的二肽衍生物的影响  

Effect of the Properties of Organic Solvents and Water Content on the Enzymatic Synthesis of Derivatives of Dipeptides Containing D-Amino Acid Residue

作　　者：沈鸿雁[1] 田桂玲[1] 闫爱新[1] 贾艳[1] 洪毅颖[1] 叶蕴华[1] 

机构地区：[1]北京大学化学与分子工程学院教育部生物有机重点实验室,北京100871

出　　处：《化学学报》2003年第7期1144-1148,共5页Acta Chimica Sinica

基　　金：国家自然科学基金(No.20242006)

摘　　要：有机溶剂性质及其水含量对酶催化合成保护的寡肽及其衍生物有显著影响.系统地研究了有机溶剂性质及在不同有机溶剂中水含量对α-胰凝乳蛋白酶催化合成含D-氨基酸残基的二肽衍生物产率的影响.用α-胰凝乳蛋白酶、枯草杆菌蛋白酶和嗜热杆菌蛋白酶在有机溶剂中催化合成了一系列保护的二肽及其衍生物,并研究了水含量对反应产率的影响,得到了十分有意义的结果.The effect of organic solvents and water content on the enzymatic synthesis of protected oligopeptides and its derivatives is very remarkable. The influence of the properties of organic solvents and water content in different organic solvents on the enzymatic synthesis of derivatives of dipeptides containing D-amino acid residue was systemically studied. alpha-Chymotrypsin, subtilisin and thermolysin were used as catalyst for the synthesis of a series of protected dipeptides and its derivatives; furthermore, the effect of water content on the enzymatic reactions was also studied.

关 键 词：有机溶剂 水含量 酶 催化合成 D-氨基酸二肽衍生物 催化活性 蛋白水解酶 

分 类 号：O629.72[理学—有机化学]