SO_2、木瓜蛋白酶致大鼠肺气肿模型的建立及应用  

Double Factors of SO_2 and Papain Stimulate to Set up Rats Pu lmonary Emphysema Model

作　　者：柴秀娟[1] 骆仙芳[2] 黄卫华[1] 朱婉萍[1] 石玲[1] 黄强[1] 蔡映云 

机构地区：[1]浙江省中医药研究院,浙江杭州310007 [2]浙江省中医院,浙江杭州310006

出　　处：《中国中医基础医学杂志》2003年第6期23-26,共4页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：浙江省中医管理局基金课题 ( 980 16 )

摘　　要：目的 :采用SO2 、木瓜蛋白酶联合造模 ,并对中药保肺定喘汤进行药效学观察。方法 :用SO2 气体定量定时刺激 ,然后再予 0 5 %木瓜蛋白酶溶液雾化吸入。观察BALF炎症细胞计数、TVL 体重比、排除 灌注体积比、单位面积肺泡数、平均肺泡面积及光镜下病理改变。结果 :模型组与健康组比较有显著性差异。用中药保肺定喘汤治疗后上述指标明显改善。结论 :SO2 、木瓜蛋白酶联合制作大鼠肺气肿模型成立 ,并可应用此模型进行相关研究。Objective:Take the double factors of SO 2 and p apain together to stimulate and set up pulmonary emphysema model,which is bette r than to set up rats p ulmonary emphysema model with one factor. Method: Make quantitative stimulation with SO 2 and papain. Result: In the model group, the rats’ weight reduced , short and quick breath, thin hair, activity markedly decreased,BALF inflammato ry cell number increased, ratio of TVL/weight rose, volume ratio of drainage /perfusion reduced, unit area pulmonary alveoli decreased, the average pulmon ary alveoli area widened, which had much difference compared with healthy group. Under the microscope,for the rats in model group, the pulmonary alveoli septu m broke, the alveoli cavity joins together,the pulmonary alveoli is obviously bigger, among which large amount of infiammatory cells infiltrated. After inter Vened by BaoFeiDingChuanTang, the data above are much improved,Conclusion: Co mbined stimulation of double factors of SO 2 and papain can establish the rats chronic obstructive pulmonary emphysema model according with pathologic changes of human being full lobular pulmonary emphysema.

关 键 词：SO2 木瓜蛋白酶 大鼠 肺气肿 动物模型 临床应用 保肺定喘汤 

分 类 号：R259.633[医药卫生—中西医结合] R285.5[医药卫生—中医内科学]