盐酸哌替啶诱发大鼠类帕金森病及中脑匀浆单胺类神经递质含量分析  

Parkinson-like disease induced by Meperidine hydrochloride and concentration of the monoamine neurotransmitters in the midbrain of rats

作　　者：魏宝强[1] 暴爱丽 李开明 牛丽凤[1] 张广新 崔培红 何晓明 苏丹[1] 

机构地区：[1]辽宁省基础医学研究所诊断学教研室,辽宁省沈阳市110101 [2]本溪市中心医院检验科,辽宁省本溪市117000 [3]辽宁省中医学院职业技术学院生理解剖教研室,辽宁省沈阳市110005

出　　处：《中国临床康复》2003年第16期2266-2267,共2页Chinese Journal of Clinical Rehabilitation

基　　金：辽宁科学技术基金资助(9810500304)~~

摘　　要：目的利用盐酸哌替啶制备类帕金森病(Parkinsondisease,PD)大鼠模型及测定其神经生化反应,探讨PD的发病机制及戒毒药物的研制。方法用高效液相-电化学检测器(HPLC-ECD)检测盐酸哌替啶、纳洛酮及溴隐亭对大鼠中脑内单胺类神经递质:去甲肾上腺素(NA)、多巴胺(DA)、高香草酸(HVA)、5-羟色胺(5-HT)、5-羟吲哚乙酸(5-HIAA)含量变化。结果(1)PD模型组(实验I组)大鼠中脑内DA,HVA,5-HT,5-HTAA含量犤(211±30),(745±199),(105±21),(13823±7295)ng/g犦与对照组比差异有显著性意义(t=2.17～3.83,P<0.05～0.01),对NA(369±82)ng/g影响不明显。(2)纳洛酮治疗组(治疗I组)DA,HVA,5-HIAA犤(5624±3323),(2275±1317),(4461±2303)ng/g犦与实验I组比差异有显著性意义(t=2.17～3.21,P<0.05～0.01)。(3)溴隐亭治疗组(治疗II组)DA,5-HT,5-HIAA犤(5468±2924),(215±59),(4935±1368)ng/g犦与实验I组比差异有显著性意义。结论盐酸哌替啶对DA神经元有选择性破坏作用。Aim To explore the pathogenesis of Parkinson's disease (PD).Methods The concentrations of monoamine neurotransmitters such as noradrenaline(NA), dopamine(DA), homovanillic acid (HVA), 5 hydroxytryptamine (5 HT), 5 hydroxyindoleacetic acid (5 HIAA) in the midbrain of rats induced by Meperidine hydrochloride, Naloxone and Bromocriptine were measured by using High Performance Liquid Chromatography Electrochemistry Device (HPLC ECD).Results (1)The concentrations of DA,HVA,5 HT,5 HTAA in the PD group were(211±30),(745±199),(105±21)and (13 823±7 295)ng/g respectively, with a significant difference between the PD and control groups (t=2.17-3.83, P< 0.05-0.01).The concentration of NA in the PD group [(369±82)ng/g] insignificantly changed.(2) The concentrations of DA,HVA,5 HIAA in the Naloxone group were (5 624±3 323),(2 275±1 371)and (4 461±2 303)ng/g respectively, with a significant difference between the PD and Naloxone groups(t=2.17-3.21,P< 0.05-0.01).(3)The concentration of DA,5 HT,5 HIAA were (5 468±2 924),(215±59) and (4 935±1 368) ng/g in the BromocriPtine group,with a significant difference between the BromocriPtine and PD groups.Conclusion Meperidine hydrochloride plays a selectively destructive role in DA neuron.

关 键 词：盐酸哌替啶 大鼠 类帕金森病 中脑 单胺类神经递质 纳洛酮 戒毒药物 含量测定 

分 类 号：R742.5[医药卫生—神经病学与精神病学]