栓线法建立大脑中动脉局灶缺血实验模型对再灌注损伤的评价意义  

Study on focal cerebral ischemia of MCA animal model induced by cylinder embolization in rats

作　　者：范红杰[1] 于维东[2] 欧阳嶷[2] 

机构地区：[1]中国医科大学附属第二医院神经内科,辽宁省沈阳市110022 [2]中国医科大学附属第一医院康复科,辽宁省沈阳市110001

出　　处：《中国临床康复》2003年第16期2292-2293,共2页Chinese Journal of Clinical Rehabilitation

摘　　要：目的为临床脑缺血研究提供更合理实用的基础研究模型。方法选用健康Wistar大鼠41只,雌雄不限,体质量250～280g,用栓线法制作大鼠大脑中动脉局灶脑缺血模型,并限定不同时间点再灌注。结果正常对照组,假手术组,缺血30min再灌注24h组未发现神经功能缺损,评分为0分。缺血90min-再灌注模型神经功能缺损评分为2.2±0.4,梗死灶部位累及皮层和基底核。缺血持续24h组评分为3.4±0.5,与缺血90min再灌注组比,t=3.797,P<0.01。结论栓线法大鼠大脑中动脉局灶脑缺血缺血90min再灌注模型更接近临床脑缺血的病程。Aim To provide the more reasonable basic animal model for clinical cerebrl ischemia study.Method MCA occlusion was made in 41 health Wistar rats by intravascular nylon filament and the different time point models of transient or permanent focal ischemia were built.Results Neurologic deficit score was 0 in control group,sham operation group and ischemia 90 min reperfusion 24 h group respectively,2.2±0.4 in ischemia 90 min reperfusion,the infracted area including both cerebral cortex and basal ganglia by HE staining,and 3.4±0.5 in ischemia 24 h group.There was a significant difference between the latter two groups(t=3.797,P< 0.01). Conclusion The model of ischemia 90 min reperfusion occlusion of MCA by cylinder embolization in rats is nearer to the clinical course of focal cerebral ischemia.

关 键 词：栓线法 局灶性脑缺血 实验 动物模型 再灌注损伤 大鼠 

分 类 号：R743.31[医药卫生—神经病学与精神病学]