汉黄芩素诱导人卵巢癌细胞A2780凋亡及对细胞端粒酶活性的影响  

Effects of Wogonin on Inducing Apoptosis of Human Ovarian Cancer A2780 Cells and Telomerase Activity

作　　者：黎丹戎 侯华新[2] 张玮 李力[1] 

机构地区：[1]广西肿瘤防治研究所临床实验中心,广西南宁530021 [2]广西医科大学化学教研室,广西南宁530021

出　　处：《癌症》2003年第8期801-805,共5页Chinese Journal of Cancer

基　　金：广西自然基金资助(桂科回0144008;桂科自0135030)

摘　　要：背景与目的:诱导肿瘤细胞凋亡和调节端粒酶的活性可能成为肿瘤治疗的一条新途径,体内、外实验表明,汉黄芩素具有抗氧化活性和抑制肿瘤细胞生长的功效。本研究旨在评价汉黄芩素诱导卵巢癌A2780细胞凋亡作用和抑制端粒酶活性的能力。方法:应用MTT法、荧光显微镜及琼脂糖凝胶电泳等方法检测汉黄芩素对人卵巢癌细胞A2780的作用;利用TRAP-ELISA方法观察药物处理前后细胞端粒酶活性的变化。结果:汉黄芩素明显抑制A2780细胞增殖,抑制作用呈时间和浓度依赖性,48h的IC50为85μg/ml;用浓度为50、100μg/ml的汉黄芩素作用A2780细胞48h,细胞出现明显的凋亡形态学改变,琼脂糖凝胶电泳出现典型的DNA梯状条带。端粒酶的活性随着汉黄芩素浓度的升高而降低;当汉黄芩素浓度大于200μg/ml时,A2780细胞端粒酶的活性受到明显的抑制。结论:50～250μg/ml汉黄芩素可抑制卵巢癌A2780细胞增殖和诱导细胞凋亡,其抗肿瘤作用机制与诱导凋亡有关,抑制端粒酶活性可能起部分作用。BACKGROUND &OBJECTIVE: Inducing apoptosis and inhibiting the telomerase activity of tumor cells became a new therapeutic means for tumor. In vivo and in vitro experiments showed that wogonin possesses antioxidant activities and inhibitory effect on tumor cells growth. This study was designed to evaluate the effect of wogonin on telomerase activity and apoptosis of human ovarian carcinoma cell line A2780. METHODS: MTT assay,fluorescent microscopy,and DNA agarose gel electrophoresis were used to determine the role of wogonin on apoptosis of A2780 cells. The telomerase activity of A2780 cells were observed by using TRAP ELASA method. RESULTS: A2780 cell growth was significantly inhibited by wogonin. The inhibiting effect showed concentration dependent and time dependent manners with IC50 of 85 μg/ml. After treatment with 50 μg/ml and 100 μg/ml wogonin for 48 hours, A2780 cells showed morphological changes associated with the characters of apoptosis under fluorescent microscope. Typical DNA ladder was found using agarose gel electrophoresis. Telomerase activity of A2780 cells was gradually decreased with the increasing of wogonin concentration. When the concentration of wogonin was higher than 200 μg/ml, telomerase activity of A2780 cells was inhibited markedly. CONCLUSION: Wogonin can inhibit proliferation and induce apoptosis of A2780 cells within a certain concentration range(50-250 μg/ml). Anticancer effects of wogonin were associated with the induction of apoptosis and partly with the suppression of telomerase activity.

关 键 词：汉黄芩素 诱导 卵巢癌 细胞端粒酶 活性 影响 肿瘤细胞凋亡 

分 类 号：R737.31[医药卫生—肿瘤]