薯蓣皂苷的次皂苷元B体外诱导人慢性髓系白血病细胞K562凋亡的研究  

Apoptosis of Human Chronic Myeloid Leukemia K562 Cell Induced by Prosapogenin B of Dioscin (P.B) In Vitro

作　　者：王三龙[1,2] 蔡兵[1] 崔承彬[1,3] 刘宏伟[4] 吴春福[4] 姚新生[4,5] 

机构地区：[1]天津生物医药研究所 [2]沈阳药科大学中药学院,辽宁沈阳110016 [3]中国海洋大学,山东青岛266003 [4]沈阳药科大学中药学院 [5]深圳中药及天然药物研究中心,广东深圳518057

出　　处：《癌症》2003年第8期795-800,共6页Chinese Journal of Cancer

基　　金：国家重点基础研究发展规划项目(973项目)(No.1998051113);国家杰出青年基金(No.39825126)

摘　　要：背景与目的:我们从福州薯蓣的根茎中首次分离得到薯蓣皂苷的次皂苷元B(P.B),并发现P.B能够显著抑制多种人肿瘤细胞的增殖。本研究的目的在于探讨P.B是否通过诱导细胞凋亡而发挥其抑制肿瘤细胞增殖的作用。方法:采用荧光及透射电子显微镜观察细胞凋亡的形态学变化;库尔特全自动颗粒粒度分析仪分析药物引起K562细胞体积大小分布的变化;琼脂糖凝胶电泳测定DNA梯状带,流式细胞术检测细胞凋亡时的变化。结果:10μmol/LP.B处理K562细胞24h时,细胞产生典型的核内染色质凝结、核片段化、细胞体积缩小及凋亡细胞表面膜出泡等形态学特征。10μmol/LP.B分别处理K562细胞6、12及24h时,能够使细胞产生明显的DNAladder和体积变化,呈一定的时效关系,并且亚G1峰的凋亡细胞随作用时间的延长而增加,分别为6.12%(6h)、35.6%(12h)和45.7%(24h)。结论:P.B可能通过诱导K562细胞凋亡而发挥其抗K562细胞增殖的作用。BACKGROUND &OBJECTIVE: The authors have isolated prosapogenin B of dioscin (P.B) newly from Dioscorea futschauensis R. Kunth (Dioscoreaceae) and found that P.B could significantly inhibit the cell proliferation of several human cancer cell lines for the first time. The objective of this paper was designed to investigate whether P.B could inhibit cancer cell proliferation by inducing apoptosis. METHODS: Morphological changes of cell nuclei were observed under invert fluorescence microscope and transmission electron microscope. The changes in the cell size distribution were analyzed by Coulter Multisizer Ⅱparticle analyzer. DNA ladder and apoptotic cells were analyzed respectively by DNA agarose gel electrophoresis and flow cytometry. RESULTS: The K562 cells treated with 10 μmol/L P.B for 24 hours showed morphological characteristics of apoptotic cells, such as decrease in cellular volume, nuclear chromatin condensation, nuclear fragmentation, and plasma membrane bleb formation. The treatment of K562 cells with 10 μmol/L P.B for 6, 12,and 24 hours caused the increase of the detected DNA ladder and the decrease of normal size cells both in a time dependent manner. The percentage of apoptotic bodies detected in the sub G0/G1 peak region by flow cytometry was also increased time dependently as analyzed as 6.12%for 6 hours, 35.6%for 12 hours and 45.7%for 24 hours treatments, respectively. CONCLUSION: These results suggested that P.B exerts its anti proliferative effect on K562 cells through inducing apoptosis of the cells.

关 键 词：薯蓣皂苷 次皂苷元B 体外诱导 慢性髓系白血病 细胞K562凋亡 研究 

分 类 号：R733.72[医药卫生—肿瘤]