k-ras、p16基因在卵巢上皮性肿瘤的表达及临床意义  

STUDY ON K-ras AND p16 IN EPITHELIAL OVARIAN CANCER AND THEIR CLINICAL SIGNIFICANCE

作　　者：王亦雄[1] 王淑玉[1] 马志松[1] 

机构地区：[1]扬州大学医学院附属医院妇产科,江苏扬州225001

出　　处：《实用临床医药杂志》2003年第3期252-254,共3页Journal of Clinical Medicine in Practice

摘　　要：目的　研究k ras、p16基因在卵巢上皮性肿瘤中的表达及其意义。方法　应用链霉菌抗生物 过氧化酶连接法检测 98例卵巢上皮癌石蜡块中的表达情况 ,应用kaplam meier法及多变量cox比例风险回归摸型 ,分析 2种蛋白表达与患者预后关系。结果　k ras蛋白表达率为 79 6% ,其表达与临床分期及病理类型有关 (P <0 0 1) ;p16蛋白表达率 60 2 % ,其表达与临床分期、癌瘤播散、淋巴转移及患者预后有关 (P <0 0 1) ;患者生存随访表明p16表达阳性者平均生存时间短于无表达者 ,k ras对生存预后影响不显著。结论　p16蛋白表达与卵巢上皮癌的恶性程度有显著相关性 ,并能判断其癌瘤播散、转移能力、估计患者的预后 ;k ras基因表达主要发生在早期或分化好的卵巢肿瘤中 ,可以作为卵巢肿瘤发生的启动基因。Objective: To investigate the expressions of k-ras and p16 in epithelial ovarian cancer and their clinical significance. Methods: Expression of k-rasp16proteinswasretros was pectively studied by immunohistochemistry, and Kaplan-Meier method and Cox′sproportional hazard regression model were used to toanalzye the relationship of their expressions with prognosis. Results: ①The expression rate of K-ras was 79 6%, and was in connection with clinical statge. ②The expression rate of p16 was 60 2% and the low expression p16 protein was significantly associated with clinical ststge, invasiveness, implantation lymph noddes metastasis, and patients′s prognosis. ③Survival fellow-up data showed that the survival time was shorter in patients with p16 expression than those without p16; and k-ras was not significant. Conclusions: P16 expession has a close relationship with malignancy degree of epithelial ovarian cancer, and significantly contributes to prognosis. The findings suggested that the postive expression of k-ras existed in earlier stages of epithelial ovarian cancer patients.

关 键 词：卵巢肿瘤 癌基因k-ras 抑癌基因P16 免疫组化 

分 类 号：R737.31[医药卫生—肿瘤]