广东株家蚕微孢子虫特异DNA片段的克隆与序列分析  

Cloning and Sequence Analysis of Nosema Bombycis's Special DNA Fragment

作　　者：邱宝利[1] 徐兴耀[2] 周鹏[3] 蔡平钟[2] 

机构地区：[1]华南农业大学昆虫学系,广州510642 [2]华南农业大学蚕业服装系,广州510642 [3]热带作物生物技术国家重点实验室,海口571101

出　　处：《华南热带农业大学学报》2003年第2期1-4,共4页Journal of South China University of Tropical Agriculture

基　　金：广东省自然科学基金(950402)项目

摘　　要：利用碱裂解方法抽提广东株家蚕微孢子虫(NosemabombycisNaёgeli,N.b.)基因组DNA,通过PCR技术扩增得到了N.b.的一段特异DNA片段。对该目的片段的序列分析发现,该特异DNA片段大小为317bp,与Malone等报道的日本株N.b.特异DNA片段大小一致,但核苷酸序列同源性仅为92.1%。The genome DNA of nosema bombycis Nageli(N.b.)was extracted by using SDS and proteinase K.A special DNA fragment was synthesized by PCR amplification.Used as the template,the special DNA fragment was cloned into T Easy Vector and transferred into E.coli DH5α,by which it could be prepared and labeled as a probe to detect N.b.in ser-iculture.The result of sequence analysis of the special DNA fragment showed that the spe-cial DNA fragment was composed of317nucleotides,similar in size to its counterpart in Japanese N.b.,but the homogeneity of Guaangdong N.b.and Japanese N.b.was only92.1%according to this317bp fragment.

关 键 词：广东株家蚕 微孢子虫 虫害防治 DNA片段 克隆 序列分析 碱裂解方法 核苷酸序列 同源性 

分 类 号：S884[农业科学—特种经济动物饲养] Q78[农业科学—畜牧兽医]