HCAP1基因对Raji细胞凋亡及凋亡相关蛋白Bax/Bcl-2的作用  

Effects of HCAP1 on apoptosis and apoptotic related proteins Bax/Bcl-2 in Raji cells

作　　者：牧启田[1] 吴向华[1] 王嵘[1] 杜均祥[1] 郭列平[1] 张海伟[2] 万大方[3] 顾建人[3] 

机构地区：[1]暨南大学医学院血液病研究所,广东广州510632 [2]暨南大学医学病理教研室,广东广州510632 [3]上海市肿瘤研究所癌基因与相关基因国家重点实验室,上海200032

出　　处：《暨南大学学报（自然科学与医学版）》2003年第4期1-5,共5页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：教育部重点科学基金 (教技司 [2 0 0 0 ] 156号 );广东省自然科学基金 ( 0 10 411);广东省医学科研基金 (A2 0 0 13 0 9)资助项目

摘　　要：目的 :研究外源HCAP1基因产物对Burkitt淋巴瘤细胞系Raji细胞凋亡及凋亡相关蛋白Bax/Bcl- 2的作用。方法 :用脂质体介导的基因转移方法 ,把外源HCAP1基因转染到Raji细胞中 ,用流式细胞术和Hochest 332 58荧光染色检测细胞凋亡 ;用Westernblot检测外源HCAP1基因对凋亡相关蛋白Bax和Bcl- 2蛋白表达的调节。结果 :外源HCAP1基因导入Raji细胞 2 4、4 8和 96h后 ,细胞凋亡率增加。Westernblot显示Bax/Bcl- 2蛋白表达比值明显增高。结论 :转染外源性HCAP1基因可诱导Raji细胞凋亡 ,使Bax/Bcl- 2蛋白表达比例上调可能是HCAP1诱导凋亡的机制之一。Aim: To investigate the effect of exogenous HCAP1 gene products on apoptosis and apoptotic related proteins Bax and Bcl-2 in Burkitt lymphoma cell line Raji. Methods: HCAP1 gene was transfected into the Raji cells by the medium of liposome. The cell apoptosis was examined by means of flow cytometry and Hochest 33258 fluorescein staining. The levels of apoptotic related proteins Bax and Bcl-2 were measured by Western blot. Results: The percentage of apoptotic cells was significantly increased in the Raji cells transfected with HCAP1, as compared with the Raji cells transfected with PBK/CMV empty vector, at 24,48 and 96 hours after transfection. Western blot showed the expression ratio of the Bax to the Bcl-2 was increased. Conclusion: Overexpression of HCAP1 gene could induce apoptosis of the Raji cells. The upergulation of the expression ratio of Bax to Bcl-2 may be involved in the pathway of apoptosis induced by HCAP1 gene.

关 键 词：HCAP1基因 RAJI细胞 细胞凋亡 Bax/Bel-2蛋白 

分 类 号：R733.7[医药卫生—肿瘤]