肠三叶因子在应激胃粘膜损伤早期修复中的作用(英文)  

Role of the ITF in early healing of stress-induced gastric lesions in rats

作　　者：聂时南[1] 钱晓明[1] 吴学豪[1] 许宝华[1] 阳世宇[1] 林欣[1] 唐文杰[1] 黄方[1] 孙海晨[1] 孙东燕[1] 李兆申[2] 

机构地区：[1]南京军区南京总医院急诊科,江苏南京210002 [2]第二军医大学长海医院消化科,上海200433

出　　处：《医学研究生学报》2003年第7期491-495,F002,共6页Journal of Medical Postgraduates

基　　金：军队"十五"医药科研基金重点项目 (批准号 :0 1Z0 5 9)

摘　　要：目的 :研究肠三叶因子 (ITF)在水浸束缚应激 (WRS)大鼠胃粘膜基因表达变化 ,探讨其在应激胃粘膜损伤早期修复作用。　方法 :采用水浸束缚应激制作模型 ,动态监测胃粘膜血流量 (GMBF) ,大体及光镜下观察粘膜损伤程度 (UI)及组织学变化 ;逆转录 多聚酶链反应 (RT PCR)检测ITF基因表达变化 ;免疫组化染色进一步证实其表达。　结果 :应激造成胃粘膜广泛损伤 ,但损伤指数在 2、4、8h逐渐减小 ,至 8h降为 6 4 .9% ,GMBF逐渐恢复 ,至 8h上升为正常 (89.8% ) ,ITF基因表达逐渐增强 (0 .0 2 2± 0 .0 0 1 )vs(0 .1 77± 0 .0 1 0 ) ,P <0 .0 1 ,免疫组化染色计分为 (0 .1 34± 0 .0 0 1 )vs(0 .2 5 3± 0 .0 1 ) ,P <0 .0 1。　结论Objectives:To determine the role of the ITF(a member of trefoil peptides) in the early phase of epithelial repairing of stress induced gastric lesion. Methods:Wistar rats were exposed to water restrait stress(WRS) for 4h,gastric mucosal blood flow(GMBF) was measured by LDF 3 Flowmeter, the extent of gastric mucosal lesions were evaluated grossly and histologically and for the expression of ITF mRNA as determined by RT PCR. To go a step further demonstration, the expression was detected by immunohistochemistry. Results:WRS applied once produced numerous gastric mucosal erosions, but the number of these lesions gradually declined and GMBF restorated at 2,4,8 h after the stress. The area of gastric mucosal lesion being reduced by 64.9% and GMBF being increased by 89.8% at 8 h, the healing of stress induced ulcerations was accompanied by the increased expression of ITF(0.022± 0.001 vs 0.177±0.010, P <0.01).The result were demonstrated further by immunohistochemistry( 0.134 ±0.001 vs 0.253±0.01, P <0.01). Conclusions: ITF may participate in the early phase of epithelial repairing known as restitution.

关 键 词：应激 胃粘膜 上皮修复 肠三叶因子 

分 类 号：R573[医药卫生—消化系统]