人回盲肠癌荷瘤裸鼠模型的建立和氟尿嘧啶的治疗作用观察  

The Chemotherapeutic Effect of Fluorouracil on lleocecal Carcinoma of Nude Mice

作　　者：袁俊[1] 郭仕英[1] 洪建军[1] 殷咏梅[2] 束永前[2] 李朝军[1] 

机构地区：[1]南京师范大学分子医学实验室,江苏南京210097 [2]南京医科大学第一附属医院肿瘤生物治疗中心,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2003年第5期450-451,F002,共3页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省卫生厅科研基金(H200110)

摘　　要：目的:探讨化疗药物氟尿嘧啶在人回盲肠癌裸鼠肿瘤治疗中的作用。方法:将人回盲肠癌细胞HCT-8接种到BALB/C雌性裸小鼠腹部皮下,按预期方案用氟尿嘧啶进行化疗。采用免疫组化SP法对瘤组织进行检测。结果:成功建立荷瘤小鼠模型。治疗后肿瘤体积无明显变化,而对照组肿瘤增长明显,且瘤组织中突变型P53蛋白的阳性率有明显下降。结论:化疗药物氟尿嘧啶对人回盲肠癌裸鼠肿瘤的治疗有一定效果,可为人肠道肿瘤的治疗提供依据。Objective: To investigate the chemotherapeutic effect of Huorouracil(5-Fu)on ileocecal carcinoma of nude mice. Methods:Human ileocecal carcinoma cells (HCT-8)were inoculated into the abdomen subcutis of BALB/C-nu/nu femal nude mice.5-Fu were administrated into the nude mice according to chemotherapeutic scheme,and the immunohistochemical method was used to examine the chemotherapeutic effect. Results: Tumors bearing model of nude mice were successfully prepared. After treatment with 5-Fu,the size of tumor was not significantly alterative,but there was outstanding advance in the control, and the expression of mutant type of P53 was decreased obviously in the carcinoma.Conclusion: 5-Fu has some efficacy in treating ileocecal tumor in model of mice, and this provides some infonnation about chemotherapy in human intestinal cancer.

关 键 词：人回盲肠癌 裸小鼠 P53蛋白 氟尿嘧啶 免疫组化 

分 类 号：R735.3[医药卫生—肿瘤] R-332[医药卫生—临床医学]