促卵泡激素对顺铂诱导卵巢癌细胞株凋亡的保护作用  

Protective Effect of Follicle Stimulating Hormone on Apoptosis of Human Epithelial Ovarian Cancer Cell Induced by Cisplatin

作　　者：黄春芳 刘东远[2] 徐卫华 沈铿[2] 

机构地区：[1]Department of Biology,College of Basic Medicine,Beijing University of Traditional Chinese Medicine [2]中国医学科学院中国协和医科大学北京协和医院妇产科,北京100730 [3]School of Life Science,University of Science & Technology of China

出　　处：《中国医学科学院学报》2003年第4期443-446,共4页Acta Academiae Medicinae Sinicae

基　　金：国家自然科学基金(39900157);国家教育委员会博士点基金(1999002310)资助~~

摘　　要：目的探讨促卵泡激素(FSH)对顺铂诱导卵巢癌细胞凋亡的保护作用。方法MTT比色法检测人卵巢上皮性癌细胞系OVCAR3转染FSH受体(OVCAR3-FSHR)细胞活性,流式细胞术分析细胞周期变化及细胞凋亡率。结果促卵泡激素能降低顺铂对细胞生长的抑制作用,同时降低S期的比例和凋亡率。结论促卵泡激素对顺铂诱导的卵巢癌细胞凋亡有保护作用。To observe the protective effects of follicle stimulating hormone(FSH)on human epithelial ovarian cancer cell apoptosis induced by cisplatin(DDP).Methods OVCAR3-FSHR cell were treated with DDP and FSH at serials of concentrations,MTT assay was used to examine the growth inhibition of OVCAR3-FSHR cell after treatment with DDP and FSH.Flow cytometry was used to analyze the change of cell cycle and percentage of apoptosis.Results It was revealed that FSH decreased the growth inhibition induced by DDP.We also demonstrated that FSH reduced the S-phase percentage compared with the DDP only groups after treatment for24hours and reduce d apoptosis percentage after48hours treatment with DDP.Conclusion It is suggested that FSH can protect the apoptosis induced by DDP.It also suggests that FSH may be an important chemoresistent reason for the chemotherapy of ovarian cancer.

关 键 词：促卵泡激素 卵巢癌 顺铂 细胞凋亡 

分 类 号：R737.31[医药卫生—肿瘤] R730.53[医药卫生—临床医学]