促卵泡激素抑制顺铂诱导的卵巢癌细胞凋亡  

Follicle Stimulating Hormone Inhibits Cisplatin Induced Apoptosis in Ovarian Cancer Cells

作　　者：黄春芳 刘东远[2] 沈铿[2] 

机构地区：[1]Department of Biology,College of Basic Medicine,Beijing University of Traditional Chinese Medicine [2]中国医学科学院中国协和医科大学北京协和医院妇产科,北京100730

出　　处：《中国医学科学院学报》2003年第4期447-450,T002,共5页Acta Academiae Medicinae Sinicae

基　　金：国家自然科学基金(39900157);国家教育委员会博士点基金(1999002310)~~

摘　　要：目的探讨促卵泡激素(FSH)对顺铂诱导卵巢癌细胞凋亡的影响及其机制。方法应用DNAFragmentation梯带电泳分析,TUNEL荧光原位凋亡检测法测定对FSH敏感的卵巢癌细胞系OVCAR3(OVCAR3-FSHR)细胞凋亡状态,应用Caspase-3活性分析法检测Caspase-3活性,RT-PCR检测凋亡相关基因survivinmRNA表达,Westernblot检测其Survivin及bcl-2蛋白表达。结果不论是DNA梯带电泳还是TUNEL荧光原位凋亡检测都表明促卵泡激素能抑制顺铂诱导的OVCAR3-FSHR细胞的凋亡。Caspase-3活性分析表明促卵泡激素能降低顺铂引起的Caspase-3活性增加。半定量RT-PCR结果表明FSH能增加survivinmRNA表达,Westernblot结果表明FSH能增加Survivin蛋白表达而对bcl-2表达无影响。结论促卵泡激素能够抑制顺铂诱导卵巢癌细胞凋亡,其作用机制可能与促进Survivin表达及降低Caspase-3活性有关。To investigate whether the follicle stimulating hormone(FSH)can inhibit apoptosis in ovarian cancer cells induced by cisplatin(DDP)and its possible mechinism.Methods DNA fragmentation assay,(TdT-mediated dUTP nick end labling TUNEL),Western blot were used to analyze the changes in expression levels of Survivin and bcl-2protein.The relative activity of caspase-3was also determined.Results200mIU/ml FSH could regulate down the percentage of apoptotic cells and DNA fragmentation induced by5.0μg/ml cisplatin,while200mIU/ml FSH increased Survivin protein expression but could' t influence the expression of bcl-2protein.Conclusion FSH can inhibit ovarian cancer cells apoptosis induced by cisplatin.The possible mechinism is up-regulation of Survivin expression and down-regulation of caspase activity.

关 键 词：促卵泡激素 卵巢癌 顺铂Sunrivin蛋白 细胞凋亡 

分 类 号：R737.31[医药卫生—肿瘤]