顺铂对人肺腺癌细胞SLC-89细胞周期、p21及c-myc表达的影响  

Effect of cisplatin on cell cycle and expression of p21 and c-myc protein of human lung adenocarcinoma SLC-89 cell

作　　者：庞荣清[1] 刘春生[2] 潘兴华[1] 王洪强[3] 赵彬[1] 吴秀琴[4] 陈志龙[1] 

机构地区：[1]成都军区昆明总医院医学实验科,650032 [2]云南省第一人民医院肿瘤外科 [3]云南省第一人民医院信息资料科 [4]成都军区昆明温泉疗养院药剂科

出　　处：《肿瘤防治研究》2003年第4期280-281,284,共3页Cancer Research on Prevention and Treatment

摘　　要：目的　观察顺铂对SLC 89细胞增殖、细胞周期、p2 1及c myc蛋白表达的影响 ,以揭示顺铂抗癌的机理。方法　运用体外细胞培养技术 ,以不同浓度的顺铂作用于培养的SLC 89细胞 72h ,分别运用MTT法测定细胞增殖和流式细胞术观察细胞周期的变化及p2 1和c myc蛋白的表达。结果　顺铂能显著抑制SLC 89细胞生长 ,72h的顺铂IC50 为 18.4 7μg/ml。顺铂能以浓度依赖方式减少S期细胞而阻滞细胞于G0 /G1期 ,并诱导细胞p2 1及c myc蛋白的表达。 结论　顺铂能显著抑制SLC 89细胞生长增殖、改变细胞周期分布并诱导细胞 p2 1及c myc蛋白的表达 ,这可能是顺铂抗癌作用的重要机理之一。Objective To investigate effect on proliferation, cell cycle and expression of p21 and c-myc protein of human lung adenocarcinoma SLC-89 cells induced by Cisplatin in order to find out the mechanism of its anticancer action. Methods By techniques of cell culture in vitro, SLC-89 cells were treated by Cisplatin in different concentration for 72h, then proliferation of cell was measured by MTT method, and cell cycle and expression of p21 and c-myc protein were observed by Flow-Cytometry(FCM) respectively. Results Cisplatin could obviously inhibit proliferation of SLC-89 cell, and IC 50 value for Cisplatin treatment at 72h was 18.47μg/ml. Cisplatin could decrease S phase cells, increase G 0/G 1 phase cells and induce the expression of p21 and c-myc protein of SLC-89 cell by concentration-dependent fashion. Conclusion Cisplatin could obviously inhibit proliferation, change cell cycle distribution and induce the expression of p21 and c-myc protein of SLC-89 cell, which may be one of important mechanisms of Cisplatin's anticancer action.

关 键 词：肺腺癌 SLC-89细胞 顺铂 细胞增殖 细胞周期 P21 C-MYC蛋白 

分 类 号：R734.2[医药卫生—肿瘤] R979.1[医药卫生—临床医学]