c-erbB2反义寡核苷酸对人卵巢癌细胞放射敏感性的影响  

Effect of c-erbB2 antisense oligodexynucleotides on radiosensitivity of human ovarian cancer cell line

作　　者：任庆兰[1] 吴永忠[1] 刘渝[1] 李少林[1] 

机构地区：[1]重庆医科大学基础医学院核医学教研室,重庆400016

出　　处：《重庆医科大学学报》2003年第4期421-423,共3页Journal of Chongqing Medical University

基　　金：自然科学基金资目 (编号 3 0 0 70 2 3 0 )

摘　　要：目的 :探讨癌基因c -erbB2的反义寡苷酸对人卵巢癌细胞放射敏感性的影响。方法 :设立c -erbB2正义寡核苷酸组和非处理组作对照 ,用RT -PCR检测卵巢癌细胞处理前后c -erbB2表达水平的变化。用MTT法及平板克隆形成试验检测人卵巢癌细胞株SKOV3在脂质体c-erbB2反义寡核苷酸作用前后受60 Coγ射线不同剂量照射后的细胞存活分数和集落形成率。结果 :c -erbB2反义寡核苷酸能抑制c-erbB2癌基因的表达 ,经脂质体介导的c-erbB2反义寡核苷酸作用的卵巢癌细胞经γ射线照射后其存活分数和集落形成率较转染正义寡核苷酸组及单纯照射组明显下降 (P <0 .0 1) ,而转染c -erbB2正义寡核苷酸组的存活分数和集落形成率与单纯照射组相比无明显差异 (P >0 .0 5 )。结论 :c -erbB2反义寡核苷酸通过抑制c -erbB2的表达降低人卵巢癌细胞株SKOV3放射抗拒性。该作用可能与c-erbB2反义寡核苷酸抑制了引起细胞辐射抗拒的细胞信号转导途径有关。Objective:To explore the effect of lipofectin-c-erbB2 antisense oligodexynucleotides on radiosensitivity of human ovarian cancer cell line.Methods:The expression of c-erbB2 was detected by means of RT-PCR;cellular response to irradiation was evaluated by MTT test and the colony forming assay.Results:Lipofectin-c-erbB2 antisense oligodexynucleotides(AS-ODN) could suppress the expression of c-erbB2,and significantly decreased the survival fraction and colony forming rate of human ovarian cancer cells after ionizing irradiation( P <0.01), while non-treament and the sense oligodexynucleotides(S-ODN) groups did not decrease on the radio-resistance level of SKOV3 cell line( P >0.05).Conclusion:c-erbB2 antisense oligodexynucleotides sensitize the SKOV3 to ionizing irradiation through decreasing the expression of c-erbB2,which might be the result of the fact that c-erbB2 antisense oligodexynucleotides inhibit the cellular signal transduction pathway relating to the radiation-resistant phenotype.

关 键 词：卵巢癌细胞株 脂质体 c—erbB2反义寡核苷酸 放射敏感性 

分 类 号：R737.31[医药卫生—肿瘤]